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Equipment:
- Hydrogen Peroxide
- Catalase
- Equally sized paper slips (a small rectangle, 2 cm X 1 cm)
- Tweezers
- Beaker
- Stopwatch
-pH testing strips
-Acids (lemon juice, vinegar, etc.)
-Bases (milk, baking soda, ammonia etc.)
For the control, take one of the paper slips, using the tweezers, and dip it into the catalase, then place the catalase covered slip at the bottom of a beaker filled about halfway with hydrogen peroxide. You cannot simply drop the slip into the beaker, you must stick the tweezers into the beaker so that they reach the bottom, and then release. When the catalase contacts the hydrogen peroxide the hydrogen peroxide will release oxygen as a reaction, these oxygen bubbles in the liquid will push the paper to the top. Start the stop watch precisely when the strip enters the hydrogen peroxide, to measure the rate of reaction, and stop it again when the slip reaches the top. And finally for the affect of pH on the enzyme, simply change the pH of the hydrogen peroxide adding an acid, or a base. Then, just rerun the experiment and it will be evident that the pH affects the rate of reaction. The pH of hydrogen peroxide is roughly 2, or in that region, so adding a base will speed up the reaction, because the pH optimum of catalase is around 7.
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∙ 12y ago
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What conditions affects the function of enzymes?
The activity of an enzyme is affected by temperature, pH and the concentration of the substrate.
What pH do neither enzymes work in?
at a ph of 13
Which organ secrets enzymes that are active at a low pH?
The stomach secretes protease enzymes that work best at a pH of around 2.
What is the enzyme's optimum pH?
I believe it's 7.3, the pH of human blood, as most enzymatic reactions occur there. However, there are special enzymes, such as the ones which are in the stomach, which work best at around a pH of 2.
Is an enzymes function dependent on temperature and pH?
Every enzymes has its own optimum pH and temperature, where it shows higher activity (pH:it is very specific; temperature:mostly not more than 30-37degree C). Above or below this situation the enzymes activity may differs exponentially due to the biochemical changes.
What are the factors affects the function of enzymes?
pH and Temperature both impact the enzyme's function.
What conditions affects the function of enzymes?
The activity of an enzyme is affected by temperature, pH and the concentration of the substrate.
Are pH level and enzymes related?
Most enzymes only work in a certain pH range; pH is not dependent on the presence of enzymes, however.
How does substrate concentration and pH affect enzymes?
Substrate concentration will affect enzymes because substrates are specific to enzymes. The pH will affect enzymes because certain enzymes will work better in certain pH levels.
How pH effects Stability and Activity of Enzymes?
Enzymes are very pH-sensitive. Any change in pH results in denaturing of proteins.
Can enzymes work over a broad range of PH levels?
Most enzymes work best at a certain pH, however there are some enzymes that can operate over a broad pH range.
What happens to enzymes at a low pH?
The activity of the enzymes depends on the specific pH needed.
What pH do neither enzymes work in?
at a ph of 13
Do you think that PH affects enzymes work and explain your answer?
yes I do,you see the enzymes are proteins that are easily denatured and the structure of an enzyme is necessary for it's work(lock and key hypothesis)so changes in pH would change it's structure causing malfunction.that why the liver and other parts of homeostasis work to maintain pH of body fluids
All enzymes function optimally at pH equals 7.4 . true or false?
Enzymes have an individual optimum pH, such as pepsin has a very low optimum pH
How pH levels affect enzymes?
It disrupts an enzymes shape and structure.
Why are enzymes mixed with vinegar less effective?
Enzymes are sensitive to changes in pH and only work with a relatively small pH range. Since vinegar is an acid, the pH of the solution containing the enzymes is changes when vinegar is added.
