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Water removes (decolorizing agent) the Malachite green from all parts of the bacteria excluding the spore. The spore appears green when examined.
Wiki User
∙ 14y ago
Wiki User
∙ 15y agothe primary stain, malachite green, is washed off with water after about 5 minutes to remove the malachite green from all of the cell's parts except the endospores
Wiki User
∙ 15y agoit just rinses the excess stain off the slide. and tap deionised water should be used, not tap water.
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What is spore production?
A spore production is the outcome of cell division. The reproduction occurs when the spores are spread by air or water.
What is required for a spore to grow into a new plant?
They need water, correct temperature (warmth), and a good location (such as in soil).
What are the seven life processes of a plant?
The life process of a plant is.... First it is either grown by a seed or a spore. Then after it gets water and sunlight it starts to grow. Then photosynthesis accursed. After that depending if it is a seed plant or a spore plant it will began to reproduce
What is the function of water pails?
It bring water for us
What is the function of niskin water sampler?
sampling water vertically.
Explain the function of Copper Sulfate in the spore staining procedure?
From what i read in my book: Because the capsule is nonionic, unlike the bacterial cell, the primary stain adheres to the capsule without binding to it. Since the capsule is water- soulube, copper sulfate, rather than water, is used to wash the purple primary stain out of the capsular material without removing the stain that is bound to the cell wall.
In spore staining what would happen it you used safarin as the primary stain and malachite green as the counterstain?
The spore would appear to be red as the safranin is heat driven into the many layers of the spore, however, as Malachite green has a weak affinity and is water soluble, it will not likely bind to the spore wall or the cell wall. You might have traces of green on the slide if any, but it will be very little. Your vegetative cells will be pink as well.
What are the different techniques used in Biology?
Gram staining: This is to determine if a bacterial cell is Gram positive or negative. This uses Crystal violet dye, Gram's iodine as a mordant, Ethyl Alcohol as a decolorization medium, and Safranin as a secondary dye. Spore staining: Primary dye is Malachite green, then slide is placed over boiling beaker, cooled, rinsed with water, then Safranin is used as a counter stain. This test is used to show whether a bacteria is a spore former. Acid fast staining: Primary dye is Carbolfuchsin, heated over beaker like the spore stain, acid alcohol is used as the decolorizing agent, and Methylyene Blue is used as a counter stain. This is used to show bacteria with acid-fast walls, which have a thick waxy lipid around them. These are the most commonly used staining techniques with Bacteria.
How spore staining method works?
Spores are impermeable structures which makes them resistant to dying. Spore staining depends on increasing the permeability of the spore coat by heating to permit the dye in. Upon cooling the dye is trapped inside the spore and not allowed out. The primary dye malachite green is a relatively weakly binding dye to the cell wall and spore wall. In fact, if washed well with water, the dye comes right out of the cell wall, however not from the spore wall once the dye is locked in. That is why there does not need to be a decolorizer in this stain. Upon further treatment with Carbol Fuchsin, the Vegetative cell walls will pick up the counterstain carbol fuchsin and will be stained pink. Spores will be of a light green colour. The identification of spores is very important for the clinical microbiologist who is analyzing a patient's body fluid or tissue since there are not that many spore-forming genera. Spores have two major pathogenic spore-forming genera, Bacillus and Clostridium in which both can cause various types of lethal diseases such as anthrax, botulism, gangrene and tetanus.
Will there be more expansion packs for Spore?
yes they have confirmed spore creature keeper which is like a alien version of sims and spore Aqua which is a water stage
What is spore production?
A spore production is the outcome of cell division. The reproduction occurs when the spores are spread by air or water.
Explain the function of the nephrons?
Nephrons basically assist the kidneys with some of its functions. Its main function is to regulate the concentration of water and sodium salts. It does this by filtering the blood.
How do you swim under water in spore hero?
you can only swim above water
Explain the importance of water to the function of the body?
Body cells can't be used if they don't have a solution to carry them. In this case, water carries the body cells.
What in the water causes brown or yellow staining of clothes after washing?
iron
Spore origins for iPod- can you go onto land?
No, sorry you can't. You only go in the water. When you do make it to land the game with that spore ends.
Congo Red Cream and Distilled Water?
The combination of Congo red cream and distilled water is used for staining cells for viewing under the microscope. Staining make the individual cells much easier to see.
