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The DNA can be denatured first then follow the annealing process by any primer and allow the extension phase to follow it and remember in this experiment the first step requires a heat resistant DNA polymerase and then do the electrophoresis and then you put the sample under the transilluminator and observe

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See visualization of nucleotide sequence site: sresearch.scienceontheweb.net/visualization.php

great visualizations of DNA sequences

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Q: How can DNA be prepared for visualization?
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