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What is the function of ethidium bromide in DNA extraction?
Ethidium bromide is a fluorescent dye that binds to DNA, allowing for visualization of the DNA under ultraviolet light during gel electrophoresis. It helps researchers to track the movement of DNA fragments in the gel and determine their sizes accurately during the DNA extraction process.
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
Does gel electrophoresis separate molecules based on their charge?
Yes, gel electrophoresis separates molecules based on their size and charge.
What color tube is drawn for a hgb electrophoresis?
A lavender-top (EDTA) tube is typically used for drawing blood samples for hemoglobin electrophoresis testing.
Which pieces of DNA will migrate to the bottom of the DNA gel first during electrophoresis?
During electrophoresis, smaller pieces of DNA will migrate to the bottom of the gel first.
Is etbr transfer with DNA during southern blotting?
The answer is NO! To me, it makes no sense, why we have to transfer etbr with DNA to a blotting membrane? When Southern blotting signals will be detected by means of Radiochemicals or fluro labelling why we have to think about EtBr?
Why is destaining done after staining in agarose gel serum electrophoresis?
Destaining is done after staining in agarose gel serum electrophoresis to remove excess stain from the gel, which can interfere with visualization of the bands. Destaining helps to improve the contrast and clarity of the bands so that they can be accurately analyzed and quantified.
What is the function of EtBr?
Ethidium Bromide is used for visualising DNA. When EtBr binds DNA it will glow pink under UV light. This allows you to take a picture of DNA bands in a gel. The gel is soaked in an EtBr solution and then lit up by UV light. Alternatly the EtBr can be incorporated into the gel beforehand but gives a poorer picture.
What is the role of formaldehyde and formamide in RNA gel electrophoresis?
Denatures the RNA
What is the role enzymes play in the procces of gel electrophoresis?
Gel electrophoresis is an analytical method used for the separation of DNA, RNA or proteins based on size.Enzymes are not requires to carry out this process
Why gel red can stain DNA fragments?
GelRed is a fluorescent dye that is designed to bind to DNA by intercalating between the base pairs. This binding causes the DNA to fluoresce under UV light, making it visible in a gel electrophoresis setting. The staining ability of GelRed allows for the visualization of DNA fragments within the gel.
What is etbr?
ETBR, or Enhanced Transmission Bulk Radio, refers to a communication technology used for high-capacity data transmission over radio waves. It often involves advanced modulation techniques and coding schemes to improve efficiency and reliability. ETBR is typically utilized in applications such as wireless broadband, satellite communications, and other fields requiring robust data transfer solutions.
Is electrophoresis part of biotechnology?
Electrophoresis is used to separate molecules based on size and charge. Since biotechnology depends on knowing what you are working with, electrophoresis of proteins, DNA and RNA is a tool used by biotechnologists.
What has the author A J Houtsmuller written?
A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins
When was Electrophoresis - journal - created?
Electrophoresis - journal - was created in 1980.
What is a common name for gel electrophoresis?
Agarose gel electrophoresis.
What has the author B J Haywood written?
B. J. Haywood has written: 'Electrophoresis - technical applications' -- subject(s): Abstracts, Bibliography, Electrophoresis 'Electrophoresis-technical application' -- subject(s): Bibliography, Electrophoresis
