Materials: a leaf, a 100 mL beaker, 10 mL ethanol, a hot plate, a power outlet to plug the hot plate into, a Petri dish, goggles, supervision (if you're a kid)
Suppose you're working with a spinach leaf. Put it into a 100 mL beaker. Add 10 mL of ethanol to it. Place the beaker with ethanol on a hot plate and set the heat to 2 or 3. Do not crush the leaf or boil the ethanol, which will turn green as chlorophyll gets extracted. Turn off the heat when the ethanol is nicely green. Let the solution cool, put the leaf in a Petri dish or discard it, and pour the chlorophyll extract someplace where you can observe it better. That's the extracted chlorophyll! You can use this method with any leaf, really. Spinach was just an example.
Grind leaves of a plant, preferably soft and very green ones - such as spinach, in a mortar and pestle with acetone (available in any pharmacy). Pour off the acetone solution which should be a dark green and contain chlorophyll. Warning - acetone is highly flammable.
Materials: a leaf, a 100 mL beaker, 10 mL ethanol, a hot plate, a power outlet to plug the hot plate into, a Petri dish, goggles, supervision (if you're a kid)
Suppose you're working with a spinach leaf. Put it into a 100 mL beaker. Add 10 mL of ethanol to it. Place the beaker with ethanol on a hot plate and set the heat to 2 or 3. Do not crush the leaf or boil the ethanol, which will turn green as chlorophyll gets extracted. Turn off the heat when the ethanol is nicely green. Let the solution cool, put the leaf in a Petri dish or discard it, and pour the chlorophyll extract someplace where you can observe it better. That's the extracted chlorophyll! You can use this method with any leaf, really. Spinach was just an example.
Cook or blend it, filter it and you are left with chlorophyll rich substance.
oil
I don't know the source of your "bright light". If it's sunlight, which of course has some UV in it, or if you take the extract into a dark room and shine a "black light" on it, you will see fluorescence. The source of the red color is chlorophyll. When in place in the leaf, the electrons of chlorophyll absorb solar energy. These excited electrons then fall into an electron transport chain that will strip them of the added energy, using that energy to form ATP and NADPH. The red color you see when you illuminate chlorophyll in a spinach extract with UV is the fall of those "excited state electrons" back into their "ground state". In falling, they shed their energy in the form of red light.
Spinach has chlorophyll (green). Watermelon has carotene(red) inside it.
Answering "Experiment on the effect of the PH of enzymatic reaction using hydrogen peroxide and extract of spinach leaf?"
because they one fungal partner which synthesis
chlorophyll is the green pigmentation in plants. Photosynthesis is the method used by plants to utilise carbon dioxide and the suns energy to form glucose to use as food other than the minerals it is able to extract from the earth.
I don't know the source of your "bright light". If it's sunlight, which of course has some UV in it, or if you take the extract into a dark room and shine a "black light" on it, you will see fluorescence. The source of the red color is chlorophyll. When in place in the leaf, the electrons of chlorophyll absorb solar energy. These excited electrons then fall into an electron transport chain that will strip them of the added energy, using that energy to form ATP and NADPH. The red color you see when you illuminate chlorophyll in a spinach extract with UV is the fall of those "excited state electrons" back into their "ground state". In falling, they shed their energy in the form of red light.
Chlorophyll, carotenoids, pheophytins and xanthophylls
it has the pigment chlorophyll in it so it can preform photosnthisis
Spinach has chlorophyll (green). Watermelon has carotene(red) inside it.
Answering "Experiment on the effect of the PH of enzymatic reaction using hydrogen peroxide and extract of spinach leaf?"
Chlorophyll is substance that is found in GREEN LEAFY VEGETABLES and not in red-coloured vegetables as chlorophyll is the major cause for the colour green in vegetables
chlorophyll a
chlorophyll
chlorophyll
Depends upon solvent used . Alcohlic extract have chlorophyll .
Organic solvents like alchohol, acetone, ether etc destroy chlorophyll. some of these solvents are used to extract chlorophyll from plant leaves.
ye you can do it. but it is happening better in blue light.