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Phenol coefficient test in microbiology?
Phenol coefficient test is best known screening test in which potency of a disinfactant is compared with that of phenol. A series of a dilutions of phenol and the disinfactant being tested are prepared. A standard amount of Salmonella tyhpii and Staphylococcus aureus are added to each dilution; the dilutions are then placed in a 20 to 37oC water bath. At 5-minute intervas, samples are withdrawn from each dilution and used to inoculate a growth medium, which is incubated for two or more days and then examined the growth. If there is no growth in the growth medium, the dilution at that particular time of sampling killed the bacteria. The highest dilution that kills the bacteria after 1o min. expousre, but not at 5min. is used to calculate the phenol coefficient. This is done by dividing the resiprocal of the appropriate phenol dilution.
Why doesn't penicillin work on all bacteria?
Penicillin is effective against bacteria that have a cell wall made of peptidoglycan. Bacteria without this type of cell wall, such as Mycoplasma and some strains of Staphylococcus, are not affected by penicillin. This is because penicillin works by disrupting the formation of the cell wall, leading to the bacteria's death.
What group of antibiotics vancomycin belongs to?
Vancomycin belongs to the class of antibiotics known as glycopeptides. These antibiotics work by inhibiting cell wall synthesis in certain bacteria, making them effective against a variety of gram-positive bacteria.
What is carbolfuchsin?
This compound is basic.
Does soda kill bacteria?
Soda does not kill bacteria. While soda may have a high sugar content or be acidic, it is not an effective disinfectant against bacteria.
What are some examples of phenol disinfectants and how effective are they in killing germs and bacteria?
Phenol disinfectants, such as Lysol and Pine-Sol, are effective in killing germs and bacteria. They work by disrupting the cell walls of microorganisms, leading to their destruction. However, it is important to follow the instructions on the product label for proper use and contact time to ensure maximum effectiveness.
How effective is phenol for cleaning surfaces?
Phenol is effective for cleaning surfaces as it has strong disinfectant properties that can kill a wide range of bacteria and viruses. However, it is important to use phenol-based products according to the manufacturer's instructions to ensure safe and effective cleaning.
Do antibiotics work against bacteria?
antibiotics are useful against bacteria because they help to kill off the nasty bacteria or they can also stop the bacteria from reproducing - so the illness doesn't get worse. this then gives your body time to make antibodies which will eventually distroy the bacteria. after this, you won't get the disease again because you are immune to it.
Why does an antibiotic not work the same for all bacteria?
Bacteria also do evolve. If one bacteria is mutated, and survives an attack by antibiotic, he multiplies and forms more bacteria which are more resistant against antibiotic. As days of surviving antibiotics and multiplying eventually creates a bacteria which is resistant against it.
What is the role of beta-mercaptoethanol in DNA extraction?
stabilization of phenol against oxidation
When are antibiotics not effective?
An antibiotic might not work because the symptoms are attributed to a virus rather than a bacterium. Antibiotics do not work against viruses.
What is the use of chick-Martin test?
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
What is the use of chick martin test?
Chick martin test is used to estimate the efficacy of a disinfectant. In this test a standard culture of Salmonella typhi, is tested for a fixed period (30 minutes) against various concentrations of phenol solution and various concentrations of the testing disinfectant; the result is expressed as the phenol coefficient, the highest dilution of the given disinfectante that kills bacteria, divided by the highest dilution of phenol that sterilizes the solution, within the measured time.
Why are antibiotics not given unless a serious infection?
Bacteria can become immune to antibiotics and the antibiotics will not work in the future when you need them. They only work against bacteria and cold and flu are caused by viruses.
Phenol coefficient test in microbiology?
Phenol coefficient test is best known screening test in which potency of a disinfactant is compared with that of phenol. A series of a dilutions of phenol and the disinfactant being tested are prepared. A standard amount of Salmonella tyhpii and Staphylococcus aureus are added to each dilution; the dilutions are then placed in a 20 to 37oC water bath. At 5-minute intervas, samples are withdrawn from each dilution and used to inoculate a growth medium, which is incubated for two or more days and then examined the growth. If there is no growth in the growth medium, the dilution at that particular time of sampling killed the bacteria. The highest dilution that kills the bacteria after 1o min. expousre, but not at 5min. is used to calculate the phenol coefficient. This is done by dividing the resiprocal of the appropriate phenol dilution.
What type of antoboitics work against viruses?
It's called NONE. Antibiotics are for bacteria. They are not for virus because they don't work on virus.
Structure of phenol?
phenol
