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im not definitely sure but in the digestive process, trypsin acts with the other proteinases to break down dietary protein molecules to their component peptides and amino acids. Trypsin continues the process of digestion (begun in the stomach) in the small intestine where a slightly alkaline environment (about p H 8) promotes its maximal enzymatic activity
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Why do you wash with PBS before trypsinisation?
Cell culture media has trypsin neutralizers, so when you wash with PBS beforehand you are washing away these factors, making trypsin active.
Why is trypsin useful during the detachment phase of cell harvesting?
trypsin breaks the chemical bonds between the cell being cultured, and the container... Trypsin is a protease that cleaves peptide chains. It is derived from a proenzyme secreted by the pancreas. It is useful in cell harvesting because it cleaves the proteins that bond cells to the walls of the petri dish or container when they are grown in vitro.
What is the difference between continuous cell lines and primary?
Primary cultures are cell cultures derived from normal animal tissue, tissues are treated with a protease such as trypsin or collagenase to breakdown the extracellualr matrix. Continous cell lines are tansformed cell lines which have lost normal apoptotic controls and are capable of indefinite growth and culture, they are usullay derived from a cancerous tumor or transformed in culture via a mutation.
Would trypsin work well in the stomach?
No. The stomach is highly acidic (~2pH) while trypsin works well in the neutral/basic environment of the small intestine.
What is a cell culture and how can a cell culture be useful to biologists?
A cell culture is where cells are grown under controlled conditions.
What is the application of trypsin?
Cell Culture
Why do you wash with PBS before trypsinisation?
Cell culture media has trypsin neutralizers, so when you wash with PBS beforehand you are washing away these factors, making trypsin active.
What pH does trypsin work at the best?
The optimal pH for trypsin is 8. It is found in the small intestine and digests proteins and polypeptides there.
How do you dilute trypsin10x?
To dilute trypsin 10x, you can combine 1 part of the 10x concentrated trypsin solution with 9 parts of a suitable diluent, such as PBS or cell culture media. For example, add 1 mL of the 10x trypsin to 9 mL of the diluent to obtain a 1x trypsin solution. Mix thoroughly before use.
Which enzymes work best in acid?
trypsin
Which protein digestive enzyme from pancreas commonly used in tissue culture technology?
Trypsin
What is tissu culture?
tissue culture you need cells from liver or organ You finely chop them and you can create a primary cell line - which still has the 3d orientation of the cell. degrade it with trypsin and you have a continuous cell line. lasts 30 passages. You can immortalize it though it loses its liver key qualities. but still remains a cell non the less. to maintain a tissue culture you need 7.4 pH Nutrience Glucose Antibiotics Serum 37 degrees
Why is trypsin useful during the detachment phase of cell harvesting?
trypsin breaks the chemical bonds between the cell being cultured, and the container... Trypsin is a protease that cleaves peptide chains. It is derived from a proenzyme secreted by the pancreas. It is useful in cell harvesting because it cleaves the proteins that bond cells to the walls of the petri dish or container when they are grown in vitro.
What is the difference between continuous cell lines and primary?
Primary cultures are cell cultures derived from normal animal tissue, tissues are treated with a protease such as trypsin or collagenase to breakdown the extracellualr matrix. Continous cell lines are tansformed cell lines which have lost normal apoptotic controls and are capable of indefinite growth and culture, they are usullay derived from a cancerous tumor or transformed in culture via a mutation.
In What organ of the digestive system does trypsin work?
Kan Madala
Would trypsin work well in the stomach?
No. The stomach is highly acidic (~2pH) while trypsin works well in the neutral/basic environment of the small intestine.
What conditions are needed for trypsin a pancreatic enzyme to work?
search it up on wikiAnswers.
