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Cell culture media has trypsin neutralizers, so when you wash with PBS beforehand you are washing away these factors, making trypsin active.

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16y ago

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Why do you wash cells in PBS?

Washing cells in phosphate-buffered saline (PBS) helps to remove any remaining culture media or serum, as well as any detached cells or debris. This step ensures that only the cells of interest are collected for downstream experiments, without any interference from external sources. Additionally, PBS provides a physiologically relevant environment that helps maintain cell viability during the washing process.


10x PBS buffer?

10X PBS (0.1M PBS, pH 7.4): NaCl --------------------------------------------- 80 g KCl----------------------------------------------- 2g Na2HPO4 (anhydrous) ----------------------- 14.4 g KH2PO4 (anhydrous) ------------------------- 2.4g Distilled water ------------------------------- 1000 ml Mix to dissolve and adjust pH to 7.4 Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.


What are the main differences between TBS and PBS in terms of programming and audience demographics?

TBS focuses on comedy and entertainment programming, while PBS offers educational and cultural content. TBS attracts a younger, more diverse audience, while PBS tends to appeal to older viewers interested in documentaries and news.


The pH of 10x PBS buffer?

The pH of 10x PBS buffer is typically around 7.4 when it is freshly prepared. It is important to note that the pH can change over time due to factors such as storage conditions and contamination. Regularly checking and adjusting the pH of the buffer is recommended for accurate results.


What is the function of PBS buffer?

PBS buffer (phosphate-buffered saline) is commonly used in biological and biochemical experiments to maintain the pH of a solution and provide essential ions for cell function. It is often used for washing cells, diluting antibodies, and preparing samples for analysis. PBS buffer helps maintain the stability and integrity of biological samples by providing a suitable environment for cells or proteins.

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