0
What else can I help you with?
Why do you wash with PBS before trypsinisation?
Washing with PBS before trypsinization helps to remove any residual medium or serum that could interfere with the trypsinization process. It helps to ensure that the trypsin can efficiently detach the cells from the culture vessel without any unwanted substances affecting the process. Additionally, washing with PBS can help to maintain the physiological pH and osmolarity of the cells during trypsinization.
What is the function of PBS buffer?
PBS buffer (phosphate-buffered saline) is commonly used in biological and biochemical experiments to maintain the pH of a solution and provide essential ions for cell function. It is often used for washing cells, diluting antibodies, and preparing samples for analysis. PBS buffer helps maintain the stability and integrity of biological samples by providing a suitable environment for cells or proteins.
What are the main differences between TBS and PBS in terms of programming and audience demographics?
TBS focuses on comedy and entertainment programming, while PBS offers educational and cultural content. TBS attracts a younger, more diverse audience, while PBS tends to appeal to older viewers interested in documentaries and news.
What is PBS in cell culture?
PBS is used as a cell culture media because every live-system nedds a ionic concentration in your environment. PBS contains phosphate and NaCl, and its a "basic" media culture, and you can add another components, like antibiotics, hormones or enzymes, for example. Remember that cells haves a membrane that exchanges substances with the environment all the time. Therefore, it needs an adjusted environment. Water can´t offer this environment to the cell growth, because you only can use distilled water, to prevent contaminations. Like this way, however, you "lost" every ion, and so the cell don´t have the "perfect" media to growth. Cell culture medias, like PBS and many others (IMDM, for example), is nothing more than distilled water added by ideal ions to the cell life. Each type of cell "prefers" a cell culture media to growth.
Why does the skin of your fingers shrink when you wash clothes for a long time '?
when we wash cloths our finger cells are dipped in hyper tonic solution they loose water by osmosis and shrink
Why do you wash cells with PBS before adding trypsin?
Washing cells with PBS helps to remove excess media, serum, and debris before adding trypsin. This helps to increase the efficiency of trypsin digestion and ensures that the trypsin can effectively detach the cells from the culture vessel. Additionally, washing the cells with PBS helps to maintain cell viability during the trypsinization process.
What is sterile pbs?
sterilized (either by filter or by heat) Phosphate buffered Saline solution used to wash cells or for other biological applications
Why do you wash with PBS before trypsinisation?
Washing with PBS before trypsinization helps to remove any residual medium or serum that could interfere with the trypsinization process. It helps to ensure that the trypsin can efficiently detach the cells from the culture vessel without any unwanted substances affecting the process. Additionally, washing with PBS can help to maintain the physiological pH and osmolarity of the cells during trypsinization.
Why Calcium and magnesium free PBS?
What cell walls are you using? In mammalian cell culture the reason you would use Ca/Mg free PBS is to help remove them from the surface they are attached to. The adherens proteins that adherent cells use to stick to whatever they are stuck to requires Ca2+ and Mg2+. Therefore if you simply want to wash the cells you have to use a buffer WITH Ca2+ and Mg2+.
What does PBS do in DNA extraction?
Phosphate Buffered Saline (PBS): a salty solution of constant pH to keep tissues, cells, and proteins intact during maceration
Who is Martha on PBS Kids?
Martha on PBS Kids is Martha on PBS Kids.
What is the function of PBS buffer?
PBS buffer (phosphate-buffered saline) is commonly used in biological and biochemical experiments to maintain the pH of a solution and provide essential ions for cell function. It is often used for washing cells, diluting antibodies, and preparing samples for analysis. PBS buffer helps maintain the stability and integrity of biological samples by providing a suitable environment for cells or proteins.
What is the function of pbs in elisa?
PBS, or phosphate-buffered saline, serves as a crucial wash and dilution buffer in ELISA (Enzyme-Linked Immunosorbent Assay) protocols. It helps maintain a stable pH and osmotic balance during the assay, ensuring that proteins and antibodies remain soluble and functional. Additionally, PBS minimizes non-specific binding, improving the specificity and accuracy of the assay results.
What is PBS Kids?
PBS Kids 900:000 AM PBS Kids Sprout (satallite and Verizon) 789:087 AM PBS 200:000 AM
When was PBS YOU created?
PBS YOU was created in 1998.
When did PBS YOU end?
PBS YOU ended in 2006.
Was Barney on PBS?
Yes and it was on PBS Kids.
