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The sequence of a DNA fragment can be determined by observing the order of the fluorescent bands on the electrophoresis gel?
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There is no answer you are all being lied to you are all in the matrix this is the matrix this is me morphius and this is the matrix do you take the blue pill or te red pill
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What is maxam Gilbert method of DNA packaging?
Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples
How can electrophoresis gels be used to implicate any suspect in a crime?
Electrophoresis per se cannot be used to implicate a suspect. Rather, electrophoresis is merely one of many analytical techniques which may be applied to a specific question, being posed by a an investigator. The value of electrophoresis is nil outside the data quality objectives (DQOs) presented by the investigating team, and the QA/QC program of the analyzing laboratory. Without QA/QC, in the lab, and without appropriate DQOs, electrophoresis is no more capable of implicating a suspect than a ouija board. Laboratories cannot perform in the real world as seen on TV's CSI, and real CSIs would go to jail if they pulled some of the stunts seen on the TV series.
Why do the number of DNA fragments and the length of each fragment produced by RFLP analysis differ from person to person?
As the DNA fragments results from the action of the restriction enzymes and on the other hand mutations alter the sites where the restriction enzymes react therefore there is difference in number and of length of each fragment from person to person.
Where do the shorter DNA bands end up reaching at the completion of gel electrophoresis?
the smallest DNA fragments are observed by a process called elcrophoresis where the DNA fragmnets placed on this gel will migrate according to their lenght so the smallest fragment will migrate the fastest and they will be found at the bottom .
What is osseous fragment in ankle bone?
what can be done if you have a osseous fragment in ankle bone
Which fragment moves the furthest in gel electrophoresis?
The smallest fragment will more furthest in the gel.
What is used to cut the DNA into fragment so its sequence could be read?
the process is called gel electrophoresis.
Will your D1S80 fragments be separated by gel electrophoresis if you have a 200 base pair and a 400 base pair?
Yes, gel electrophoresis separates fragments based on their size. Therefore it will be able to separate a 200bp fragment from a 400bp fragment provided you use the correct gel composition (as this affects the sensitivity to size differences).
Analysis of DNA fragments in gel electrophoresis involves what?
i)- the size of the DNA fragment, ii)- the density of the agarose gel, iii) - the intensity of the migratory electric field.
How does gel electrophoresis is used to make a DNA fingerprint?
Gel electrophoresis separates DNA fragment on the basis of their size. In DNA fingerprinting or DNA typing given sample is cut up with restriction enzymes and run through electrophoresis and results are analyzed to check for DNA polymorphism between the given sample and a sample form suspect. In nutshell gel electrophoresis is boon for the people in forensics.
What is maxam Gilbert method of DNA packaging?
Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples
How can electrophoresis gels be used to implicate any suspect in a crime?
Electrophoresis per se cannot be used to implicate a suspect. Rather, electrophoresis is merely one of many analytical techniques which may be applied to a specific question, being posed by a an investigator. The value of electrophoresis is nil outside the data quality objectives (DQOs) presented by the investigating team, and the QA/QC program of the analyzing laboratory. Without QA/QC, in the lab, and without appropriate DQOs, electrophoresis is no more capable of implicating a suspect than a ouija board. Laboratories cannot perform in the real world as seen on TV's CSI, and real CSIs would go to jail if they pulled some of the stunts seen on the TV series.
What agilent bioanalyzer does and its advantages over traditional gel electrophoresis?
Agilent bioanalyzer uses a microchannel based electrophoresis cell that allow rapid and sensitive investigation of nucleic acid sample.advantages: many samples types can be analyzed on bioanalyzer, including total RNA, labeled RNA, small and micro RNAs, and small and large DNA fragment.
What information can you determine from gel electrophoresis?
You can estimate the concentration of DNA or RNA in your extract as well as the size of the fragment(s) isolated by comparing to a known molecular marker/DNA ladder. 2 strand are smaller than 3 strand
What does the accuracy of the fragment sizes tell you about the resolving ability of agarose-gel electrophoresis?
The larger fragements will not be very accurate because they cannot resolve in high consentrations of the agarose in the gel. The percent of agarose in the gel affects the ability to resolve larger fragements of DNA
Is 'A year that was' a sentence or a fragment?
Fragment.
Does a single band form in electrophoresis gel if all the DNA fragment is of same size?
if all the fragments are of same size (same fragment length), it will form a single band on an agarose gel. unless you are using other gel electrophoresis technique like DGGE, then only it will separate fragments with different sequences, but that will depends on the resolution power too.
