promoter
according to information from http://www.rothamsted.ac.uk/notebook/courses/guide/trans.htm " if the RNA polymerase attaches to a special sequence called a promoter, an additional small protein, the factor sigma, will also attach to the polymerase and lock it on the DNA. The factor 'sigma' will only attach itself to the complex DNA / RNA polymerase when the RNA polymerase is attached to a promoter. Another hypothesis is that the factor sigma attaches to RNApol anyway and the enzyme is then able to slide along the DNA until it finds a promoter. It prevents detaching and speeds up promoter location, and decreases the affinity of RNApol for general regions of DNA. " Therefore, the answer seems to be, RNA attaches to DNA through a small protein called the factor sigma once the RNA polymerase attaches itself to a chain sequence called a "promoter". according to information from http://www.rothamsted.ac.uk/notebook/courses/guide/trans.htm " if the RNA polymerase attaches to a special sequence called a promoter, an additional small protein, the factor sigma, will also attach to the polymerase and lock it on the DNA. The factor 'sigma' will only attach itself to the complex DNA / RNA polymerase when the RNA polymerase is attached to a promoter. Another hypothesis is that the factor sigma attaches to RNApol anyway and the enzyme is then able to slide along the DNA until it finds a promoter. It prevents detaching and speeds up promoter location, and decreases the affinity of RNApol for general regions of DNA. " Therefore, the answer seems to be, RNA attaches to DNA through a small protein called the factor sigma once the RNA polymerase attaches itself to a chain sequence called a "promoter". role of sigmaActually RNA Polymerase can bind to DNA anywhere in the entire genome but sigma factor attaches to polymerase only when it is at promotor. sigma factor dissociates when polymerase crosses promotor. sigma factor stablises the pre initiatiation complex. Actually there are many promoter and many genes but which gene to be transcribed is decided by sigma factor.
DNA Polymerase III is responsible for adding new nucleotides to the strand being synthesised. Also involved in DNA replication are DNA Polymerase I which replaces primers with nucleotides, and DNA Ligase which joins fragments of DNA together.
The enzyme that transcribes the DNA into RNA is called RNA polymerase.
RNA Polymerase
by the proof-reading activity of DNA polymerase enzyme
kinase
according to information from http://www.rothamsted.ac.uk/notebook/courses/guide/trans.htm " if the RNA polymerase attaches to a special sequence called a promoter, an additional small protein, the factor sigma, will also attach to the polymerase and lock it on the DNA. The factor 'sigma' will only attach itself to the complex DNA / RNA polymerase when the RNA polymerase is attached to a promoter. Another hypothesis is that the factor sigma attaches to RNApol anyway and the enzyme is then able to slide along the DNA until it finds a promoter. It prevents detaching and speeds up promoter location, and decreases the affinity of RNApol for general regions of DNA. " Therefore, the answer seems to be, RNA attaches to DNA through a small protein called the factor sigma once the RNA polymerase attaches itself to a chain sequence called a "promoter". according to information from http://www.rothamsted.ac.uk/notebook/courses/guide/trans.htm " if the RNA polymerase attaches to a special sequence called a promoter, an additional small protein, the factor sigma, will also attach to the polymerase and lock it on the DNA. The factor 'sigma' will only attach itself to the complex DNA / RNA polymerase when the RNA polymerase is attached to a promoter. Another hypothesis is that the factor sigma attaches to RNApol anyway and the enzyme is then able to slide along the DNA until it finds a promoter. It prevents detaching and speeds up promoter location, and decreases the affinity of RNApol for general regions of DNA. " Therefore, the answer seems to be, RNA attaches to DNA through a small protein called the factor sigma once the RNA polymerase attaches itself to a chain sequence called a "promoter". role of sigmaActually RNA Polymerase can bind to DNA anywhere in the entire genome but sigma factor attaches to polymerase only when it is at promotor. sigma factor dissociates when polymerase crosses promotor. sigma factor stablises the pre initiatiation complex. Actually there are many promoter and many genes but which gene to be transcribed is decided by sigma factor.
kinase
the RNA polymerase attaches to the promoter and transcribes the gene in messenger RNA, or mRNA
DNA Polymerase III is responsible for adding new nucleotides to the strand being synthesised. Also involved in DNA replication are DNA Polymerase I which replaces primers with nucleotides, and DNA Ligase which joins fragments of DNA together.
The double strand helix is opened by enzymes called helicase and this allow the RNA polymerase to copy the DNA strand. The double strand helix is opened by enzymes called helicase and this allow the RNA polymerase to copy the DNA strand.
The process wherein messenger RNQ (or mRNA) is given a message is called transcription. In this process, n mRNA molecule is made (or transcribed) using DNA as the template. Essentially, the nucleotide sequence on a gene is read by an enzyme called RNA polymerase which synthesizes the mRNA molecule. Put simply, RNA polymerase scans the length of DNA until a gene is encountered. When the enzyme reaches the correct position, it begins adding complimentary nucleotides to make the mRNA molecule. This way, the entire gene is transcribed and copied on to the mRNA molecule.
There is no single enzyme responsible for DNA duplication. But the most important ones are:Helicase: it unwinds the DNA helixRNA polymerase: adds the RNA primerDNA polymerase: adds the complementary strand of DNALigase: attaches the DNA fragments together
The enzyme that transcribes the DNA into RNA is called RNA polymerase.
The step of protein synthesis that occurs in the nucleus is transcription. Transcription is the process of copying the genetic code from DNA into RNA. The RNA molecule that is produced during transcription is called messenger RNA (mRNA). mRNA then travels to the cytoplasm, where it is translated into a protein. In transcription, the DNA double helix opens up at a specific location called the promoter. A protein called RNA polymerase binds to the promoter and begins to move along the DNA strand, unzipping the helix as it goes. RNA polymerase then uses the exposed DNA strand as a template to synthesize a complementary RNA strand. This RNA strand is the mRNA molecule. Once the mRNA molecule is complete, it separates from the DNA and travels to the cytoplasm. In the cytoplasm, the mRNA molecule binds to a ribosome. The ribosome then reads the mRNA molecule and uses the information to assemble a protein molecule. The protein molecule is then released from the ribosome and can go on to perform its function in the cell. Here are the steps of transcription in detail: Initiation: RNA polymerase binds to the promoter region of DNA. Elongation: RNA polymerase moves along the DNA strand, unzipping the helix and synthesizing a complementary RNA strand. Termination: RNA polymerase reaches a termination sequence in the DNA and stops synthesizing RNA. The mRNA molecule that is produced during transcription is a single-stranded molecule that contains the same information as the original DNA strand. However, the mRNA molecule is modified in a few ways. First, the 5' end of the mRNA molecule is capped with a group of nucleotides called a 5' cap. The 5' cap helps to protect the mRNA molecule from degradation and helps it to bind to the ribosome. Second, the 3' end of the mRNA molecule is polyadenylated, which means that it is added to a long chain of adenine nucleotides. The polyadenylation tail helps to stabilize the mRNA molecule and helps it to be transported to the cytoplasm. Transcription is a complex process that is essential for protein synthesis. It is the first step in the process of converting genetic information into a functional protein molecule.
RNA Polymerase
by the proof-reading activity of DNA polymerase enzyme