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What else can I help you with?
What is the use of primer in DNA replication?
DNA polymerase requires a primer because it can't initiate polymerization by it self only,but requires a preexisting free 3'OH group to which it can add deoxynucleotides forming phosphodiester bond & the free 3'OH group is provided by the primer.Therefore the DNA polymerase requires primer.
What are the 3 rules that DNA replication must follow?
DNA cannot replicate de novo (i.e. there must be a free 3' OH group to attach nucleotides to) Replication must go from 5' to 3' The two strands of DNA are antiparallel Complementary base pairing
Why DNA pol needs 3'OH to attach new nucleotide?
DNA polymerase requires a 3'OH group to attach a new nucleotide because it catalyzes the formation of a phosphodiester bond between the incoming nucleotide and the existing DNA chain. The 3'OH group provides the necessary chemical linkage for the new nucleotide to join the DNA chain during replication.
The process of DNA replication necessary before a cell?
when DNA replicates they can be separated by the enzyme helicase single strand DNA binding a proteins.one DNA stranderd incorsed liding strands which form 5 to 3 primates now there is no problem . but lagging strand makes a problem from 5 primes to 3 primes too. it forms a pieces called okazaki fragments. first the RNA primase lays down a RAN primer and then DNA polymerase 3 lays down new DNA. the process the RNA primers with DNA. finally DNA ligase links with okazaki fragments.
How do cells copy themselves?
Oh, dude, it's like a whole cellular soap opera! So, basically, cells go through this process called DNA replication where they unzip their double helix DNA, make a copy of each strand, and then zip it back up. It's like the ultimate DNA photocopying machine, but way more complex and way less boring. So yeah, cells just casually copy themselves like it's no big deal.
What end of the DNA molecule allows the best access for DNA polymerase during replication?
3'OH end 3'OH end
Why RNA-primer only initiates replication?
Laxu's answer made no sense so I deleted it. Next time Laxu please contribute something that makes some grain of sense. Only RNA primers can be used because DNA polymerase cannot add nucleotides without an existing 3-OH' to build on. RNA can be added to an existing template without this requirement and is then used by the DNA polymerase to initiate the strand.
In DNA structure the 3' end terminates with?
In DNA structure, the 3' end terminates with a hydroxyl group (-OH). This -OH group plays a crucial role in DNA replication as it is where new nucleotides are added by DNA polymerase, forming a phosphodiester bond.
Why only RNA primer provide 3-OH' end for DNA replication?
The reason you only need RNA primer at the end to make a DNA replication is because it has a free 3' OH. RNA is ribonucleic acid. RNA contains long chains of nucleotides.
What is the use of primer in DNA replication?
DNA polymerase requires a primer because it can't initiate polymerization by it self only,but requires a preexisting free 3'OH group to which it can add deoxynucleotides forming phosphodiester bond & the free 3'OH group is provided by the primer.Therefore the DNA polymerase requires primer.
What are the 3 rules that DNA replication must follow?
DNA cannot replicate de novo (i.e. there must be a free 3' OH group to attach nucleotides to) Replication must go from 5' to 3' The two strands of DNA are antiparallel Complementary base pairing
Why are RNA primers needed?
RNA primers are used to initiate the DNA replication at the template strand. DNA molecules require a free 3' OH, to which it could add the nucleotides. This free 3' OH is provided by the RNA primer. So prior to the synthesis of DNA a short fragment of RNA is synthesized that is later excised and filled with DNA molecules.
What serves as the template for DNA replication?
The eukaryotic chromosomes are larger in size. Hence in order to overcome the limitation of DNA synthesis, multiple origins of replication are present in order to complete replication in a reasonable period of time.At the replication origin the strands of DNA must dissociate and unwind in order to allow access to DNA polymerase.Unwinding of the duplex at the origin as well as along the strands as the replication process proceeds is carried out by helicases.The resultant regions of single-stranded DNA are stabilized by the binding of single-strand binding proteins.The stabilized single-stranded regions are then accessible to the activities of other enzymes required for replication to proceed.The site of the unwound template strands is termed the replication fork.In order for DNA polymerases to synthesize DNA they must encounter a free 3'-OH which is the substrate for attachment of the 5'-phosphate of the incoming nucleotide.During replication the 3'-OH is supplied through the use of an RNA primer, synthesized by the primase activity. The primase utilizes the DNA strands as templates and synthesizes a short stretch of RNA generating a primer for DNA polymerase.Synthesis of DNA proceeds in the 5'---->3' direction through the attachment of the 5'-phosphate of an incoming deoxy nucleosidetriphosphate (dNTP) to the existing 3'-OH in the elongating DNA strands with the concomitant release of pyrophosphate.Initiation of synthesis, at origins of replication, occurs simultaneously on both strands of DNA.DNA synthesis process then proceeds bidirectionally, with one strand in each direction being copied continuously and one strand in each direction being copied discontinuously.The experimental support for this bidirectional mode of replication in the cells of higher eukaryotes was acquired by the fiber autoradiography of labeled DNAs got from mammalian cell cultures. When the replicating DNA extracted and isolated from eukaryotic cells are examined under the electron microscope, the clear "bubble" like structures extending from multiple replication origins are clearly visible.source: www.examville.com
Why DNA pol needs 3'OH to attach new nucleotide?
DNA polymerase requires a 3'OH group to attach a new nucleotide because it catalyzes the formation of a phosphodiester bond between the incoming nucleotide and the existing DNA chain. The 3'OH group provides the necessary chemical linkage for the new nucleotide to join the DNA chain during replication.
When and how does replication happen?
Oh, dude, replication happens during the S phase of the cell cycle, where DNA unwinds and duplicates itself. It's like making a photocopy of a really important document, but in this case, it's your genetic material. So, yeah, replication is basically your cells being all like, "Let's make some copies of ourselves, because why not?"
What is the function of primers?
RNA primers are used to initiate the DNA replication at the template strand. DNA molecules require a free 3' OH, to which it could add the nucleotides. This free 3' OH is provided by the RNA primer. So prior to the synthesis of DNA a short fragment of RNA is synthesized that is later excised and filled with DNA molecules.
The process of DNA replication necessary before a cell?
when DNA replicates they can be separated by the enzyme helicase single strand DNA binding a proteins.one DNA stranderd incorsed liding strands which form 5 to 3 primates now there is no problem . but lagging strand makes a problem from 5 primes to 3 primes too. it forms a pieces called okazaki fragments. first the RNA primase lays down a RAN primer and then DNA polymerase 3 lays down new DNA. the process the RNA primers with DNA. finally DNA ligase links with okazaki fragments.
