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Simple or gram? Simple uses methylene blue, gram uses crystal violet

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14y ago

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What color is simple staining on a bacillus and coccus using methylene blue stain?

Methylene blue stains everything blue.


What does the simple stain tell us about the bacteria?

The result of simple staining of microorganisms is that they can be identified and studied under a microscope.


Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin Through the microscope the green structures are?

Bacillus cells stained with malachite green and safranin will appear red under the microscope due to the counterstaining with safranin. Malachite green primarily stains the spores of Bacillus while safranin stains the rest of the cell, resulting in red-stained vegetative cells and green-stained spores.


What is the bacteria mophology for bacillus subtilis by simple stain?

B. subtilis is indeed a Gram positive rod.However, there is confusion as to whether it is an obligate aerobe or a facultative anaerobe. Although classed as an obligate aerobe, it has been shown to grow under strict anaerobic conditions.


What is a simple stain reagent?

A simple stain reagent is a type of stain, such as crystal violet or methylene blue, that is used to colorize cells or tissues for microscopic examination. These stains bind to cells and provide contrast to make them visible under a microscope. Simple stains are a quick and easy method to visualize the basic morphology and structure of cells.


What strain of plague do fleas carry?

Fleas carry the bacterium Yersinia pestis, formerly known as Pasteurella pestis. The plague bacillus can be stained with Giemsa stain and typically looks like a safety pin under the microscope.


What is the function of a stain in microscope work?

As the cells are transparent, the components cannot be seen clearly through a microscope. We stain the cell so that we can see the components of the cell clearly through a microscope.


What type of charge does a simple stain have?

A simple stain has a positive charge.


What are the acid-fast stain results for Bacillus subtilis?

Bacillus subtilis is a Gram-positive bacterium and does not typically show acid-fast staining results. This means that it does not retain the stain when subjected to the acid-fast staining procedure commonly used to detect mycobacteria.


What structure in the cheek cells was stained the darkest?

When stained with iodine and observed under a compound microscope the nucleus will stain the darkest. The cheek cell is frequently employed here as they are simple to collect and tend to take the stain easily.


Why is a stain called a stain?

It's simple, because it's a stain.


Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?

There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.