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Enzymes are biological catalysts which cause the rate of a reaction to increase (by providing an alternate reaction pathway with a lower activation enthalpy). They are very specific to the reaction they catalyse.
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3 variables that can cause an enzyme to lose its ability to function?
An enzyme is a catalyst for chemical reactions. Three variables that can cause an enzyme to lose its ability to function are temperature, pH level and concentration.
What factors affect the rate of enzyme activity?
-Heat, temperature, &pHtemperature, pH level, concentration of enzyme, concentration of substrate( my sec.1 science book)
Which chemical test shows the presence of an enzyme in a biological washing powder?
biurets test is the biochemical test for enzymesan enzyme is a protein and as the biochemical test for proteins is biurets,, its the same
What can Tobin conclude about the relationship between the enzyme concentration and the reaction rate in the presence of excess substrate?
There is a direct relationship; as the enzyme concentration increases, the rate of reaction increases.
What does citrate permease do?
Citrate Permease is an enzyme that is responsible for transporting citrate inside the cell. Some bacteria have this enzyme while others do not. You can test for the presence of citrate permease by performing a Simmon's Citrate biochemical test.
What are the 3 things that can affect the enzymes and how?
Temperature, presence of acid, presence of base as all three can cause denaturation of enzyme.
Can the presence of inhibitors or activitors affect enzyme activity?
yes, enzymes can be inhibited by other enzymes.
What happens when activation energy is in presence of an enzyme?
the energy decreases
What can cause an enzyme to lose its confirmation?
When a protein is denatured, this can cause an enzyme to lose its confirmation.
The presence of a repressor protein prevents the action of what enzyme?
RNA polymerase.
What would be the likely outcome if you increased the concentration of substrate for an enzyme in the presence of a noncompetitive inhibitor?
No change in enzyme activity would be observed.
What are some factors that could impact the function of an enzyme?
This depends on the enzyme. Temperature, pH, and the presence of co-factors, or allosteric inhibitors.
Why cant the ONPG test be substituted for the determination of lactose fermentation?
ONPG test detects only presence of beta galactosidase enzyme whereas lactose fermentation requires the presence of permease as well as beta galactosidase enzyme.
What are three factors that control the rate of enzyme reaction?
Presence of:Competitive inhibitorsNon-competitive inhibitorsAllosteric sitesNegative feedback inhibitionIncrease/decrease of enzyme/substrateCooperativity
Can enzyme cause diseases?
Yes
What can cause an enzyme to be denatured?
Heat
3 variables that can cause an enzyme to lose its ability to function?
An enzyme is a catalyst for chemical reactions. Three variables that can cause an enzyme to lose its ability to function are temperature, pH level and concentration.
