vital dye in microbiology
it is a basic dye that will stain the cells. That makes it a positive stain.
To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Using two different dye
because the outer membrane is phospholipid bi layer, which is positive charged one.
Pink Dye
Malachite green
it depends on the animal
Methylene blue
Usually, it does stain.
An organic stain is considered a biological stain using organic dye. Organic dyes used are often modified from the textile industry.
methane blue
it is a basic dye that will stain the cells. That makes it a positive stain.
To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
coomassie brilliant blue dye is a linear dye whereas the silver stain dye is a nonlinear one. Besides that coomassie dye has a lower sensitivity and is used for detection of highly abundant proteins. Silver stain relies on adsorption rather than absorption and is more suitable for detection of low abundant proteins but with a higher sensitivity.
a dye is used on inanimate objects & stain is used on animate objects .
chromatography.