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How are DNa bands viewed?
DNA bands are usually visualized using techniques such as agarose gel electrophoresis or polyacrylamide gel electrophoresis. After electrophoresis, DNA bands can be viewed under UV light by staining the gel with a fluorescent dye, such as ethidium bromide. The DNA bands will appear as distinct bands of varying sizes depending on the migration pattern of the DNA fragments.
What do dark bands in the chromosome indicate?
When DNA coils into a chromosome, it keeps its shape with the help of associated proteins. Where the DNA is very tightly coiled, and the proteins very condensed, that region appears as a dark band (G-band). G-bands are not very rich in genes, which means that it's ok that the DNA is tightly coiled, because it does not have to be very accessible anyway. Note that these bands are only visible when certain types of dye are used. Other types of dye will case gene-rich regions to stain darker and gene-poor regions to stain more brightly. See also: Chromosomes: Organization and Function By Adrian Thomas Sumner, 10.2.2
What is acetocarmine stain?
Acetocarmine stain is a biological dye used in microscopy to highlight cell nuclei. It binds to DNA and RNA, making the nuclei appear red or pink under a microscope. It is commonly used in plant and animal cytology studies.
Which is an example of a dye used in a simple stain?
Methylene blue
What dye is used to stain cheek epithelial cells in lab?
methane blue
What region of the DNA does the giemsa stain dye?
Giemsa stain binds the regions with high content of adenine-thymine complex.
How are DNa bands viewed?
DNA bands are usually visualized using techniques such as agarose gel electrophoresis or polyacrylamide gel electrophoresis. After electrophoresis, DNA bands can be viewed under UV light by staining the gel with a fluorescent dye, such as ethidium bromide. The DNA bands will appear as distinct bands of varying sizes depending on the migration pattern of the DNA fragments.
What is done to the DNA to make the bands visible in gel electrophoresis?
In gel electrophoresis, DNA is treated with a dye that binds to the DNA molecules, making them visible as bands under ultraviolet light.
What kind of dye is used to make a negative stain and why?
Pink Dye
What do dark bands in the chromosome indicate?
When DNA coils into a chromosome, it keeps its shape with the help of associated proteins. Where the DNA is very tightly coiled, and the proteins very condensed, that region appears as a dark band (G-band). G-bands are not very rich in genes, which means that it's ok that the DNA is tightly coiled, because it does not have to be very accessible anyway. Note that these bands are only visible when certain types of dye are used. Other types of dye will case gene-rich regions to stain darker and gene-poor regions to stain more brightly. See also: Chromosomes: Organization and Function By Adrian Thomas Sumner, 10.2.2
What is acetocarmine stain?
Acetocarmine stain is a biological dye used in microscopy to highlight cell nuclei. It binds to DNA and RNA, making the nuclei appear red or pink under a microscope. It is commonly used in plant and animal cytology studies.
What is nucleaer staining?
A stain selective for cell nuclei, usually based on the binding of a basic dye to DNA or to nucleohistone.
Which is an example of a dye used in a simple stain?
Methylene blue
What dye is used to penetrate and stain an endospore?
Malachite green
Why do the nuclei stain darker than the cytoplasm?
Nuclei stain darker than the cytoplasm in microscopy because they contain higher amounts of DNA, which binds more of the dye used for staining. This results in a more intense coloration in the nucleus compared to the cytoplasm, which has a lower concentration of DNA.
How do you used tie dye?
you fold up the piece of clothing and put a couple of rubber bands around it in different places and just dye the piece and when you are done, remove the rubber bands and you have your tie dye.
What is the role of EtBr in electrophoresis?
Ethidium bromide interchalates with DNA. It doesn't affect electrophoresis, but it help visualise the DNA bands after electrophoresis. The EtBr that is bound to the DNA will fluoresce under ultraviolet light.
