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If alcohol (decolorizing step) is omitted then the primary stain absorb by the bacteria will not be washed away. This will result in all or nearly all the bacteria to appear purple in color under the microscope.

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Why slide need to soak in alcohol before staining?

Soaking the slide in alcohol before staining helps to remove any dirt, debris, or contaminants that may be present on the slide. This ensures that the stain can properly adhere to the slide and provide accurate results during the staining process.


Is staining a material a physical change?

Staining is a chemical process.


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What happens if you drink alcohol in the desert?

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What are staining artifacts?

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What is the staining process for separating bacteria?

One common staining process for separating bacteria is the Gram staining method. This involves applying crystal violet, iodine, alcohol, and safranin to the bacterial sample. Gram-positive bacteria will retain the crystal violet stain, appearing purple, while Gram-negative bacteria will not retain it and will appear pink after the safranin counterstain.


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What is the grammatical name of if process is omitted?

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