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What if You did not apply heat during the application of the primary stain?
Wiki User
∙ 12y ago
if you didnt apply heat or Clorox, then the stain would not go away completely and we all don't want that!
Wiki User
∙ 12y ago
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Assume that during the performance of this exercise you made several errors in your spore staining procedure?
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.
Why must heat be used with application of the primary stain during acid fast staining?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
Why is it essential that the primary stain and the counterstain be of contrasting colors?
It is essential that primary stain and the counterstain be of contrasting colors so that the target of the primary stain can easily be differentiated on a contrasting background.
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
What is the purpose of crystal violet in the Gram's stain procedure?
Its the primary stain of the procedure. IT stains the Gram positive organisms
Assume that during the performance of this exercise you made several errors in your spore staining procedure?
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.
What is the application of heat not required during the ap plication of the counterstain in acid fast stain?
17
Why must heat be used with application of the primary stain during acid fast staining?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
Why is it essential that the primary stain and the counterstain be of contrasting colors?
It is essential that primary stain and the counterstain be of contrasting colors so that the target of the primary stain can easily be differentiated on a contrasting background.
Can you apply oil based deck stain over acrylic stain?
Yes.
Can you revive dried out wood stain by adding paint thinner?
No, I have tried this and the end result you get is a very washed out blotchy stain that is very hard to apply. You can try bleaching the stain, and re-apply a stain that has a very close color that can mimic the stain you already have.
How do you apply semitransparent deck stain?
Use a roller or staining pad to apply the oil or water-based semi-transparent stain. Go in the direction of the grain and do not allow the stain to puddle.
Do you apply the stain before or after assembling the furniture?
For a seamless finish, apply the stain before assembling the furniture.
What is the best way to stain bathroom floor cabinets?
The best way to stain bathroom floor cabinets is to first sand the wood. After that, apply the wood conditioner, then the stain and finally apply the finish.
Are of application?
To stain or dye tissues (animals or plants).
Can you apply a ureathane over deck stain?
No, you should not apply urethane over deck stain. You should sand down the old deck stain first before applying any other type of finish.
Can you use a sponge to apply deck stain to vertical surfaces?
You can use a sponge applicator on a broom handle or extension
