0
It removes the crystal violet (primary stain) from gram negative bacteria. It does not remove Crystal violet as easily from gram positive bacteria, because the highly peptidoglycan walls of gram positive bacteria interact with crystal violet and iodine to form a strong bond (CV-Iodine complex). So, gram positive cells hold on to the stain instead of letting it wash away.
Wiki User
∙ 14y ago
Wiki User
∙ 9y agoIn any staining procedure, when one forgets to decolorize or rinse, there may be too much primary stain or background. The net effect is to reduce contrast levels, which is the whole purpose of staining in the first place.
Wiki User
∙ 9y agoFailure to apply the decolorizer in a gram stain all cells will appear purple since the crystal violet stains both gram positive and gram neg cells and the safranin is not likely to be seen over the darker crystal violet. Failure to add iodine in a gram stain will affect probable decolonization of gram positives and no noticeable effects on gram negatives.
Wiki User
∙ 12y agogram pos and gram neg would turn pink
Wiki User
∙ 12y agoethyl alcohol
Wiki User
∙ 13y agoIt messes it up.
Add your answer:
Earn +20 pts
What are the reagents and steps min a Gram stain?
The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
List the steps of the gram-staining procedure in order and fill in the color of gram-positive cells and gram-negtive cells after each step?
____________Color of Gram-positive_______Color of Gram-negativePrimary stainCrystal violet_____Purple____________________ PurpleMordant:Iodine____________Purple________________________PurpleDecolorizing agent:Alcohol-acetone__ Purple____________________ ColorlessCounterstain:Safranin___________Purple_________________________Red
How can you remove crystal violet in a Gram Stain?
You won't be able to positively distinguish gram negative from gram positive organisms. Crystal violet is used first so that gram positive organisms will take up the dye and this color is not affected by safrinin later on. Gram negative organisms will lose the purple coloring during the decolorizer step, therefore need to be counterstained so that you can view the organisms.
What part of bacterial cell is most involve with gram staining and why?
In the gram staining process, gram positive bacteria appear to be purple because their cell walls, which contain a large concentration of peptidoglycans, are strongly dyed. Gram negative bacteria appear pink because their walls asborb less dye. This occurs becayse there is a smaller concentration of peptidoglycans and an additional lipid layer surrounds the cell wall. ANSWER The exact mechanism of action of this staining technique is not clearly understood. However, it is known that differences in the biochemical composition of bacterial cell walls parallel differences in their Gram-stain reactions. Gram-positive bacterial walls are rich in tightly linked peptidoglycans (protein-sugar complexes) that enable cells to resist decolorization. Gram-negative bacterial walls have a high concentration of lipids (fats) that dissolve in the decolorizer (alcohol, acetone, or a mixture of these) and are washed away with the crystal violet. The decolorizer thus prepares gram-negative organisms for the counterstain.
What are the reagents and steps min a Gram stain?
The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
List the steps of the gram-staining procedure in order and fill in the color of gram-positive cells and gram-negtive cells after each step?
____________Color of Gram-positive_______Color of Gram-negativePrimary stainCrystal violet_____Purple____________________ PurpleMordant:Iodine____________Purple________________________PurpleDecolorizing agent:Alcohol-acetone__ Purple____________________ ColorlessCounterstain:Safranin___________Purple_________________________Red
How can you remove crystal violet in a Gram Stain?
You won't be able to positively distinguish gram negative from gram positive organisms. Crystal violet is used first so that gram positive organisms will take up the dye and this color is not affected by safrinin later on. Gram negative organisms will lose the purple coloring during the decolorizer step, therefore need to be counterstained so that you can view the organisms.
What is stained with primary dye?
Bacterial smears that are undergoing some type of differential staining method are stained with a primary dye. Primary dye typically refers to a Crystal-voilet stain that is used in the gram staining procedure to color all cells purple. After a mordant (iodine-makes the dye complex less soluble), decolorizer, and a counterstain are added to the primary dye, a person can view the slide under a microscope in order to distinguish gram-positive bacteria from gram-negative bacteria. The cell wall of gram-negative bacteria will not retain the primary dye and be washed away with the decolorizer. Gram-neg. bacteria will then take on the counterstain providing contrast between the two types of bacteria when viewed under a microscope.
What part of bacterial cell is most involve with gram staining and why?
In the gram staining process, gram positive bacteria appear to be purple because their cell walls, which contain a large concentration of peptidoglycans, are strongly dyed. Gram negative bacteria appear pink because their walls asborb less dye. This occurs becayse there is a smaller concentration of peptidoglycans and an additional lipid layer surrounds the cell wall. ANSWER The exact mechanism of action of this staining technique is not clearly understood. However, it is known that differences in the biochemical composition of bacterial cell walls parallel differences in their Gram-stain reactions. Gram-positive bacterial walls are rich in tightly linked peptidoglycans (protein-sugar complexes) that enable cells to resist decolorization. Gram-negative bacterial walls have a high concentration of lipids (fats) that dissolve in the decolorizer (alcohol, acetone, or a mixture of these) and are washed away with the crystal violet. The decolorizer thus prepares gram-negative organisms for the counterstain.
What is the difference between Jensen's version ang hucker's modification in gram staining based on reagents and gram reaction to fungi?
What is modified in Jensen's modification of Gram stain? Jensen's modification: This method involves use to methyl violet as primary stain, iodine and potassium iodide in water as mordant, absolute alcohol as decolorizer and neutral red as counterstain
Is gram staining related to flagella?
No. It is a staining on the cell itself.
Who developed Gram staining?
Gram staining was devised by Hans Christian Gram of Denmark in the 1800s. (1853-1938)
How do you make decolorizer for gram stain?
It is an alchoholic compound i.e. 95% ethanol.
Why is decolorization necessary when performing a gram stain?
During the procedures of a gram stain, decolorization is necessary to remove any stain or color from the gram negative cells. When a dye is used to stain gram positive cells, both gram positive and gram negative cells retain color. Mordant is used to bind the original stain to gram positive cells so when decolorizer is used they retain color. After the mordant has been used a decolorizer is used to wash away colo in gram negative cells. Counterstains are used to stain gram negative cells to better visualize contrasting cells. An example of a decolorizer that works well is ethanol.
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
