Alsalam alkom
There is a difference between the two term (lawn culture and streak culture), if you used a loop for making new culture from primary culture you called that streaking method (like ABC streaking method), while if you used swab and streaked almost all the plate then, you can say (lawn culture)..
In conclusion to the above
1- streaking is used to purify the bacterial culture (i.e to make well isolated colony)
2- lawn culture is used for antibiotic sensitivity tests
Ali Maki .
It depends, if there is no growth or colony appearance on streak line and only it shows growth in b/w the streak line then it is certainly a contamination and if there are colonies on streak line and not ressemble with the streak culture then also it is a contamination but there can be a chance that colony appears due to some fault in streaking procedure and the inoculum drops between the streak line so it depends.
To obtain a pure culture, a technique called streak plate method is commonly used. This technique involves streaking a sample on an agar plate in a way that isolates individual colonies, allowing for the growth of pure cultures. Subsequent subculturing from a single isolated colony can help to ensure a pure culture.
In scientific circles, the streak plate method is considered to be a rapid qualitative isolation method. To be effective, one must reduce the number of organisms in the inoculums by spreading a loop of culture over an agar plate. This ensures that individual cells are properly separated on the surface for the purpose of differentiating various species. The method is as follows: Using a sterile loop, microbes are initially transferred to the plate with one swipe. On the subsequent swipes, the loop is heated in the flame of a Bunsen burner to lessen the population of microbes being transmitted. Streak patterns are also done in via T-streak or by applying the loop to four quadrants of the plate.
A streak plate technique is used to isolate individual bacterial colonies on a solid agar plate to obtain pure cultures, while a serial dilution technique is used to dilute a bacterial sample in a series of steps to obtain a range of concentrations for further analysis. Streak plate technique is qualitative, focusing on colony isolation, while serial dilution technique is quantitative, focusing on estimating bacterial concentration.
A streak plate with two species of bacteria will show separate colonies with distinct morphologies and colors. Each species will grow in its own isolated area on the plate, allowing for differentiation between them. It is important to observe and document the characteristics of each colony to identify and classify the bacteria present.
A streak plate is used to determine the streak colour of a mineral. You take the mineral and scratch it against the streak plate, and it will create a coloured streak on the plate. The streak is not always the same colour as the mineral itself.
The lack of a streak would indicate that the mineral is harder than the streak plate, or the color of the streak is the same as the color of the streak plate.
Diamond will not leave a streak on a porcelain streak plate because diamond is harder than the streak plate. It will leave a scratch on the streak plate for the same reason.
When performing a streak plate culture, the nutrient plate should be rotated in a clockwise or counterclockwise direction to create isolated colonies on different parts of the plate. The rotation ensures that the inoculating loop spreads the bacteria evenly across the agar surface, leading to distinct colonies for further analysis.
It depends, if there is no growth or colony appearance on streak line and only it shows growth in b/w the streak line then it is certainly a contamination and if there are colonies on streak line and not ressemble with the streak culture then also it is a contamination but there can be a chance that colony appears due to some fault in streaking procedure and the inoculum drops between the streak line so it depends.
"What is the difference between single plate clutch and multi plate clutch?"
I do not have a picture, but when you scratch aquamarine on a streak plate, the powder will be white colored.
Two minerals that do not leave a streak on a streak plate are quartz and fluorite. Both minerals have a hardness higher than that of the streak plate, so they will not leave a streak when rubbed against it.
Hematite is typically a dark gray to black mineral with a reddish streak, while galena is a silvery-gray mineral with a cubic crystal structure. One way to differentiate between them is by performing a streak test: hematite will leave a reddish-brown streak, while galena will leave a gray streak. Additionally, galena is heavier than hematite and has a metallic luster.
a streak test is a test wheree you rub a mineral across a streak plate to see the color of its streak, which is a better indentifying factor of the mineral than the external color. A scratch test is when you scratch a mineral to find out its hardness on the Mohs Scale of Hardness. This is also another useful identifying factor
You can determine the streak of a mineral whose Mohs scale is higher than the streak plate by either filing or crushing with a hammer before rubbing the sample on a streak plate.
The powder left behind on a streak plate is called a streak. It is produced by scraping a mineral sample across the plate to reveal its color and texture. This streak can be compared to a mineral's known streak color to help with identification.