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What is the name of the enzyme responsible for adding nucleotides to the 3' end of a growing DNA strand?
The enzyme responsible for adding nucleotides to the 3' end of a growing DNA strand is called DNA polymerase.
How are nucleotides added to the 3' end of a DNA strand during replication?
During DNA replication, nucleotides are added to the 3' end of a DNA strand by an enzyme called DNA polymerase. This enzyme attaches new nucleotides to the existing strand in a complementary manner, following the base pairing rules (A with T, and G with C). The 3' end of the DNA strand provides a free hydroxyl group (-OH) that allows the DNA polymerase to add the new nucleotide, extending the DNA strand in the 5' to 3' direction.
Does the 3' or 5' end of a DNA helix grow?
The 3' end of a DNA helix grows during DNA replication. The enzyme DNA polymerase adds nucleotides to the 3' end of the growing DNA strand.
What is the significance of the 3' end of DNA in the process of DNA replication?
The 3' end of DNA is important in DNA replication because it serves as the starting point for the addition of new nucleotides during the synthesis of a new DNA strand. The enzyme responsible for adding new nucleotides can only attach them to the 3' end of the existing DNA strand, so the presence of a free 3' end is crucial for the replication process to proceed smoothly.
How does the process of DNA synthesis proceed from 5' to 3' direction?
During DNA synthesis, new nucleotides are added to the growing DNA strand in a specific direction, from the 5' end to the 3' end. This is because DNA polymerase, the enzyme responsible for synthesizing DNA, can only add new nucleotides to the 3' end of the existing strand. As a result, DNA synthesis proceeds in a 5' to 3' direction.
What is the name of the enzyme responsible for adding nucleotides to the 3' end of a growing DNA strand?
The enzyme responsible for adding nucleotides to the 3' end of a growing DNA strand is called DNA polymerase.
What is a sticky end?
A Sticky End, referring to Biology is recombinant DNA. After DNA has been cut by a restriction enzyme it has "sticky ends" or recombinant DNA at the ends.
What is the function of primer in PCR?
it synthesizes a single RNA primer at the 5' end of the leading end.
What is main function of DNA polymerase?
The DNA polymerase enzyme synthesises the complementary DNA strand to a single stranded DNA strand (in vivo and in vitro). This often requires the presence of a 3' end for the polymerase enzyme to bind to before synthesis can begin. Taq polymerase (A DNA polymerase) is often used in PCR reactions to synthesise DNA in vitro using primers to provide a 3' end to bind to.
How are nucleotides added to the 3' end of a DNA strand during replication?
During DNA replication, nucleotides are added to the 3' end of a DNA strand by an enzyme called DNA polymerase. This enzyme attaches new nucleotides to the existing strand in a complementary manner, following the base pairing rules (A with T, and G with C). The 3' end of the DNA strand provides a free hydroxyl group (-OH) that allows the DNA polymerase to add the new nucleotide, extending the DNA strand in the 5' to 3' direction.
Does the 3' or 5' end of a DNA helix grow?
The 3' end of a DNA helix grows during DNA replication. The enzyme DNA polymerase adds nucleotides to the 3' end of the growing DNA strand.
What happens when a restriction enzyme is used on DNA?
when restriction enzyme is use on DNA basically it just first losen up the DNA, usally DNA is coiled, and so the restriction enzyme jsut breka the DNA and leave a sticky end, so that it can be put back together, the cell have to be able to do that because in nature, that's the way for cell to stop protein production and the cell still need that gene
How is DNA made in your bodies?
DNA is made in the body through a process called DNA replication. This involves the separation of the two strands of DNA, followed by the synthesis of two new complementary strands using the existing strands as templates. The enzyme DNA polymerase plays a key role in catalyzing the formation of new DNA strands.
What is the significance of the 3' end of DNA in the process of DNA replication?
The 3' end of DNA is important in DNA replication because it serves as the starting point for the addition of new nucleotides during the synthesis of a new DNA strand. The enzyme responsible for adding new nucleotides can only attach them to the 3' end of the existing DNA strand, so the presence of a free 3' end is crucial for the replication process to proceed smoothly.
How does the process of DNA synthesis proceed from 5' to 3' direction?
During DNA synthesis, new nucleotides are added to the growing DNA strand in a specific direction, from the 5' end to the 3' end. This is because DNA polymerase, the enzyme responsible for synthesizing DNA, can only add new nucleotides to the 3' end of the existing strand. As a result, DNA synthesis proceeds in a 5' to 3' direction.
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
How does DNA go from 5' to 3' during the process of replication?
During DNA replication, the enzyme DNA polymerase adds new nucleotides to the growing DNA strand in a specific direction, from the 5' end to the 3' end. This is because DNA polymerase can only add nucleotides to the 3' end of the existing strand, resulting in the new strand being synthesized in the 5' to 3' direction.
