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The DNA polymerase enzyme synthesises the complementary DNA strand to a single stranded DNA strand (in vivo and in vitro). This often requires the presence of a 3' end for the polymerase enzyme to bind to before synthesis can begin. Taq polymerase (A DNA polymerase) is often used in PCR reactions to synthesise DNA in vitro using primers to provide a 3' end to bind to.
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What enzymes are responsible for replication of the DNA molecule?
Helicase is the enzymes that splits the double helix into two separate strands, and DNA Polymerase (as opposed to RNA Polymerase) joins the nucleotides together in the new strands being created.
Can the DNA polymerase editing function remove DNA adducts?
No, DNA polymerase does not have the ability to remove DNA adducts. Adducts are chemical modifications that occur on the DNA molecule, and they typically require specialized DNA repair enzymes to be removed. DNA polymerase is primarily responsible for synthesizing new DNA strands during replication and repair processes.
What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
What is a limitation of DNA polymerase?
A key limitation of DNA polymerase is that it can only add nucleotides to an existing strand of DNA; it cannot initiate the synthesis of a new strand from scratch. This means that a primer, typically made of RNA, must be present for DNA polymerase to start DNA synthesis. Additionally, DNA polymerase has a proofreading function, but it can still make errors during replication, leading to potential mutations.
How does DNA polymerase function in DNA replication?
DNA Polymerase III is responsible for adding new nucleotides to the strand being created. DNA Polymerase I replaces the primers with DNA nucleotides. The fragments are then joined together by ligase, and a new strand has been created.
How does DNA polymerase function in the process of transcription?
DNA polymerase does not function in the process of transcription. Transcription is the process where RNA is synthesized from a DNA template by RNA polymerase. DNA polymerase, on the other hand, is involved in DNA replication, where it synthesizes a new DNA strand using a DNA template.
What function does a DNA polymerase have?
To split DNA strands to create doubles.
What specific component does DNA polymerase III require in order to function correctly?
DNA polymerase III requires a primer, which is a short piece of RNA or DNA, in order to function correctly.
What is the name of the two enzymes that help in the duplication of DNA?
More than two enzymes are involved. However, the main ones are DNA Polymerase I and DNA Polymerase III. DNA Polymerase III adds new nucleotides and DNA Polymerase I removes primers.
Dna polymerase has a what?
DNA polymerase has a proofreading function that helps to ensure accuracy during DNA replication by checking for errors and correcting them in real time.
Which describes the main main purpose of RNA polymerase during transcription?
RNA polymerase produce mRNA from DNA
What enzyme elongates DNA?
DNA polymerase is the main enzyme responsible for elongating DNA strands during DNA replication. It catalyzes the addition of nucleotides to the growing strand in a 5' to 3' direction.
Does RNA polymerase require helicase for its function?
No, RNA polymerase does not require helicase for its function. RNA polymerase is responsible for synthesizing RNA from a DNA template, while helicase is an enzyme that unwinds the DNA double helix during processes like DNA replication and transcription.
What enzymes are responsible for replication of the DNA molecule?
Helicase is the enzymes that splits the double helix into two separate strands, and DNA Polymerase (as opposed to RNA Polymerase) joins the nucleotides together in the new strands being created.
Can the DNA polymerase editing function remove DNA adducts?
No, DNA polymerase does not have the ability to remove DNA adducts. Adducts are chemical modifications that occur on the DNA molecule, and they typically require specialized DNA repair enzymes to be removed. DNA polymerase is primarily responsible for synthesizing new DNA strands during replication and repair processes.
What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
What enzyme is used to join DNA nucleotides?
In DNA replication, DNA polymerase III is the enzyme which joins the DNA nucleotides together via phospodiester bonds.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.
