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Microaerophiles are microorganisms which are unable to grow when oxygen concentrations reach those found in air (20%) but nevertheless whose growth requires the presence of some oxygen (e.g., 2 to 10%). "Microaerophiles appear to grow best in the presence of a small amount of free oxygen. They grow below the surface of the medium in a culture tube at the level where oxygen availability matches their needs
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Which type of organism aerobe anaerobe microaerophile facultative anaerobe would most likely be affected negatively by the use of old media?
I would think an aerobic organism would do the worst in an oxygen poor media, which would be one of the results of having an older media sitting around.
What is the difference between a faculatative anaerobe and a microaerophile?
A faculatative anerobe does NOT require oxygen for growth but may use if available so it can grow in aerobic and anaerobic conditions. Too much oxygen is NOT toxic. A micro-aerofile CAN'T grow without oxygen but too much oxygen is toxic so it must have a low concentration in order to grow.
Where would you expect to see growth of a strict aerobe anaerobe microphile and facultative anaerobe in the fluid thioglycollate medium?
The sodium thioglycollate in the broth creates a redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.Fluid thioglycollate broth also typically contains a redox potential indicator such resazurin, which produces a pink color in an oxidized environment. As with the BHI media, organisms will only be able to grow where their oxygen requirements are met, and will localize to the area(s) of their oxygen requirements in the fluid thioglycollate broth.Obligate aerobes will grown on or very close to the top (high oxygen). Obligate anaerobes will only grow on the bottom (no oxygen). Facultative anaerobes will grow throughout but more on top. Microaerophiles will be found in a band where only a small amount of oxygen is found. They would be close to but not on the top.
How microbes are classified based on their oxygen preferences?
On the basis of oxygen requirement microorganisms are classified asAerobes: organisms that use molecular oxygen as electron acceptor.Anaerobes: organisms that use some molecule other than molecular oxygen as electron acceptor.Facultative organisms : organisms that can use either molecular oxygen or some other chemical compound as electron acceptor.
Use of agar slant?
An agar slant is when a test tube is filled with liquid agar and allowed to cool and harden at an angle (slant). Agar is mixed with other nutrients to provide a medium for which bacteria can grow on.
What would you call a microaerophile that needs an elevated carbon dioxide level?
Capnophile.
Is plasmodium falciparum aerobic or anaerobic?
It is an obligate microaerophile. Meaning it lives best in small amounts of oxygen.
Which type of organism aerobe anaerobe microaerophile facultative anaerobe would most likely be affected negatively by the use of old media?
I would think an aerobic organism would do the worst in an oxygen poor media, which would be one of the results of having an older media sitting around.
What is the difference between a faculatative anaerobe and a microaerophile?
A faculatative anerobe does NOT require oxygen for growth but may use if available so it can grow in aerobic and anaerobic conditions. Too much oxygen is NOT toxic. A micro-aerofile CAN'T grow without oxygen but too much oxygen is toxic so it must have a low concentration in order to grow.
Where would you expect to see growth of a strict aerobe anaerobe microphile and facultative anaerobe in the fluid thioglycollate medium?
The sodium thioglycollate in the broth creates a redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.Fluid thioglycollate broth also typically contains a redox potential indicator such resazurin, which produces a pink color in an oxidized environment. As with the BHI media, organisms will only be able to grow where their oxygen requirements are met, and will localize to the area(s) of their oxygen requirements in the fluid thioglycollate broth.Obligate aerobes will grown on or very close to the top (high oxygen). Obligate anaerobes will only grow on the bottom (no oxygen). Facultative anaerobes will grow throughout but more on top. Microaerophiles will be found in a band where only a small amount of oxygen is found. They would be close to but not on the top.
How microbes are classified based on their oxygen preferences?
On the basis of oxygen requirement microorganisms are classified asAerobes: organisms that use molecular oxygen as electron acceptor.Anaerobes: organisms that use some molecule other than molecular oxygen as electron acceptor.Facultative organisms : organisms that can use either molecular oxygen or some other chemical compound as electron acceptor.
Use of agar slant?
An agar slant is when a test tube is filled with liquid agar and allowed to cool and harden at an angle (slant). Agar is mixed with other nutrients to provide a medium for which bacteria can grow on.
