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The main disadvantage: it cannot be used to identify hookworm eggs. The other main argument against the Kato technique is that it is messy and therefore exposes technicians to an unnecessarily high risk of infection.Advantages of the Kato thick smear method include: it detects low infections which may be missed by the brine flotation method; it gives a higher egg count even in light infections; it is easy to prepare and this method involves less expense.
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What is the disadvantage of having a really thick smear when staining?
Having a really thick smear when staining can result in uneven staining, making it difficult to differentiate between different cell types or structures. It can also lead to overlap of cells, obscuring details and making it harder to analyze the sample. Additionally, a thick smear may take longer to dry, increasing the risk of artifacts or distortion in the stained cells.
What will happen when a thick smear is stained and viewed under a microscope?
When a thick smear is stained and viewed under a microscope, individual cells will be harder to distinguish due to overlapping. This can make it challenging to see details within individual cells, such as the internal structures or boundaries. It is typically recommended to use a thin smear for better visualization and interpretation of cellular details.
Is there any difference if the prepared smear is too thick or too thin?
Thin smears of blood are needed to investigate hematological problems or disorders of the blood. It is also used to identify the parasite within the blood. Thick films enables the microscopist to screen the blood of a larger volume. They are more sensitive than the thin film.
In gram staining why will very thick smears give inaccurate results?
If a smear exhibits uneven thickness, overlapping cells may not get the proper exposure to the reagents. This results in uneven or mottled staining. For example, in the thicker areas of the smear, gram-negative cells may not decolorize sufficiently and end up staining purple.
Why is it important to limit the quantity of cells used to prepare smear?
By applying to many cell in a smear it would become difficult to study the morphology of single cell as they will form clumps for e.g. if we want to study some cocci then it would be difficult to estimate there occurence i.e. either they are in chain or in cluster or may be diplococci or monococci.
Advantage of kato thick smear technique?
Katothick faecal smear
What is the disadvantage of having a really thick smear when staining?
Having a really thick smear when staining can result in uneven staining, making it difficult to differentiate between different cell types or structures. It can also lead to overlap of cells, obscuring details and making it harder to analyze the sample. Additionally, a thick smear may take longer to dry, increasing the risk of artifacts or distortion in the stained cells.
Why are thick or dense smears less likely to provide a good smear preparation?
because if too much smear the sample will look to indistinct
What would be observed in the blood smear of patient who has malaria?
sBoth thick and thin blood smears are taken from the finger tip of earlobe of malaria patient. This smear is kept in water to dissolve and remove the haemoglobin from the slide. The slide is stained and visualised under oil immersion lens to see the malaria parasites directly.
What is the thick and thin line technique?
Thick and thin line technique is an enhancement technique commonly used by Graphic Designers. For this technique, thin lines are used in between adjoining surfaces. Thick lines are suitable when only one side can be seen. The drawing illustrates the idea.
What will happen when a thick smear is stained and viewed under a microscope?
When a thick smear is stained and viewed under a microscope, individual cells will be harder to distinguish due to overlapping. This can make it challenging to see details within individual cells, such as the internal structures or boundaries. It is typically recommended to use a thin smear for better visualization and interpretation of cellular details.
Is there any difference if the prepared smear is too thick or too thin?
Thin smears of blood are needed to investigate hematological problems or disorders of the blood. It is also used to identify the parasite within the blood. Thick films enables the microscopist to screen the blood of a larger volume. They are more sensitive than the thin film.
What is the name of the technique where you apply thick paint?
impasto
What advantage does thick scaly skin have?
the thick scaly skin would be strong to defend against attacks
What kind of art technique uses a thick watercolor combined with chalk?
Bodycolor or Gouache
Why is it important not to use thick or dense bacterial smears?
In a thick smear the bacteria will be too concentrated, reducing the amount of light passing through the slide, the stain may not penetrate adequately, and it will be difficult to visualize individual cells.
Which one has the greater mechanical advantage a thick wedge or a thin wedge?
A thick wedge has a greater mechanical advantage than a thin wedge. The mechanical advantage of a wedge is calculated by dividing the length of the slope by the thickness of the wedge. Therefore, a thicker wedge will have a larger mechanical advantage since it has a longer slope relative to its thickness.
