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An inoculating loop has a small circular ring at its tip and is used to collect cultures that are based in liquid. On the other hand, an inoculating needle can extract microorganisms from solid or dense material so they can be transferred to another location like a petri dish.
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∙ 9y ago
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∙ 12y agoa loop will make the surface area a lot bigger for microorganisms to attach to, in comparison with a needle's point. also, needled tend to destroy the surface of the agar and may make the microorganism colony structure uneven for characterization.
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∙ 10y agoAn inoculating needle is used on solid media because the culture is more dense as well as the fact that the smaller tip of the needle allows you to pick up bacteria from a single CFU (colony forming unit) while avoiding contamination from other colonies on the same solid media. With liquid media, there is no distinction between the different colonies so a loop can be used, as well as the fact that it is easier to pick up liquid with a loop than a needle.
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∙ 13y agoinocultion needle is used for retrieving solid or dense media and the inoculation loop is used to retrieve liquid media.
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∙ 11y agothey are used to injection of the microscopic organism
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∙ 12y agounclear question .
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Why you should avoid allowing the loop filled with inoculate from touching the lip of either the source bottle or the bottle to be inoculated?
To avoid cross-contamination, and indeed all other forms of contamination.
To use a needle and cotton?
to use needle and cotton means to knit
Flaming an inoculating loop?
There is no "set" time limit. For proper aseptic technique, you should wait until the inoculating loop/needle is visibly red so that all organisms are dead, etc.
What is the term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium?
inoculation
What is an inoculation?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
How do you inoculate a solid media?
To inoculate a solid media, a sterilized inoculation loop or needle is used to pick up a small amount of the desired culture. This culture is then streaked onto the surface of the solid media in a specific pattern to ensure isolated colonies grow. The inoculated media is then incubated at the appropriate temperature to allow the colonies to grow.
Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
Why the loop is flamed after completing the inoculation?
because when we inoculate the culture with wire loop,some organism may be sticked to it which may be harmful or can cause trouble.so to destroy the remaining organisms wire loop is flamed again.
Why do you use an inoculating loop instead of a needle to make the transfer from the culture plates to the culture tubes?
Because the solid media is more dense over a smaller area so a inoculating needle is used to retrieve the specimen. Where as for a liquid medium the specimen is more spread out over the liquid. The inoculating loop can collect more liquid because there is more metal present at the inoculating specimen retrieval point and has the ability to collect liquid in the loop. I'm currently taking general microbiology and my lab book hardly covers this. A.C.
Why does a straight inoculating needle used to inoculate an agar deep tube?
because you want to introduces as little air as possible
How do you do a slip stitch?
Insert the knitting needle into the loop as if to knit (as opposed to the direction you insert to purl), slip the loop onto the inserted needle without creating a new stitch.
What way should the needle's loop hole face on a tattoo machine?
it is like a vibrating needle :3
In subculturing when do you use the inoculating loop?
Se utiliza una vez el asa de siembra o la aguja se encuentra visiblemente roja, de manera que mueran todos los organismos. De esta manera poder esparcir a su vez la muestra en la laminilla.Translation: It is used once the loop or needle is visibly red, so that all organisms die. Thus turn to spread the sample on the slide.
How inoculating needle use?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
What is the purpose of flaming the loop before and after use?
Inoculating loop is used to inoculate microbial colony or sample on culture medium and to avoid the undesired microbial cells or to avoid contamination flaming of inoculating loop is necessary it is also called as incerination.
What are the difference between a wire loop and a wire needle?
ewan ko
Why is it necessary to flame the loop between cultures?
because when we inoculate the culture with wire loop,some organism may be sticked to it which may be harmful or can cause trouble.so to destroy the remaining organisms wire loop is flamed again.
