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Pectinase is an enzyme that breaks down pectin which is found in plant cell walls. Used in degradation of plant materials, such as speeding up the extraction of fruit juice from fruits. Also pectinases have also been used in wine production.
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Is nad plus a substrate or product?
Substrate.
Is glucose a product or substrate?
both
Why you are using enzyme blank and substrate blank instead of using a single blank to find out the enzyme activity?
I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high. How can I troubleshoot high enzyme blank values in Pectinase assay ?
If Pectinase only breaks down pectin and It has no effect in cellulose What term is used to describe this characteristic of enzymes?
Enzymes are substrate specific; meaning that their active site only allows for a certain substrate to bind - in this case, pectin, and the enzyme has no effect on any other biological molecules
What is an example of a substrate?
Your Mother
Is NAD a substrate or a product?
Substrate.
Is nad plus a substrate or product?
Substrate.
Is glucose a product or substrate?
both
Who discovered pectinase?
pectinase is first isolated by Henri Braconnot
Why you are using enzyme blank and substrate blank instead of using a single blank to find out the enzyme activity?
I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high. How can I troubleshoot high enzyme blank values in Pectinase assay ?
How does pH affect pectinase?
The optimum pH for pectinase is 4, if the ph goes either above or below this the pectinase becomes denatured
How does pectinase affect the environment?
Pectinase will digest pectin, if there is any around.
Is nadh substrate or product?
Reactant
If Pectinase only breaks down pectin and It has no effect in cellulose What term is used to describe this characteristic of enzymes?
Enzymes are substrate specific; meaning that their active site only allows for a certain substrate to bind - in this case, pectin, and the enzyme has no effect on any other biological molecules
Is pectinase a monomer or a polymer?
Pectinase is an amino acid polymer, aka a protein.
What is an example of a substrate?
Your Mother
What is the substrate of phosphorylase?
The substrate is Glucose-1-phospate which is broken down by only Phosphorylase and produces Starch as its end product
