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what kind of controls are run in the expirement? why are they important? could others be used? (PCR lab)

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11y ago

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What is a common name for gel electrophoresis?

Agarose gel electrophoresis.


How to read an electrophoresis gel effectively?

To read an electrophoresis gel effectively, start by identifying the direction of the gel and the size markers. Then, analyze the bands on the gel, noting their size, intensity, and position. Compare the bands to known standards or controls to interpret the results accurately.


How does gel electrophoresis work and what kind of information can you determine from gel electrophoresis?

Gel electrophoresis separates DNA or proteins based on size and charge by applying an electric field to move molecules through a gel matrix. Smaller molecules move faster and thus travel further in the gel. Gel electrophoresis can be used to determine the size, quantity, and purity of DNA fragments or proteins, as well as for DNA fingerprinting and genetic testing.


What gel is typically used in electrophoresis experiments?

The gel typically used in electrophoresis experiments is agarose gel.


What was used before gel electrophoresis?

Before gel electrophoresis, techniques like paper electrophoresis and agarose slab gel electrophoresis were used for separating and analyzing DNA or proteins. These methods were less efficient and had lower resolution compared to gel electrophoresis.


Can a gel electrophoresis be performed both horizonnally and vertically?

yes for example 2D gel electrophoresis


What is used to sort DNA into different lenghts?

Gel Electrophoresis


Where can one learn more on gel electrophoresis?

To learn more about gel electrophoresis, one can Google it. There is also a whole Wikipedia article dedicated to gel electrophoresis, and it happens to be quite informative.


What causes the absence of bands in gel electrophoresis?

The absence of bands in gel electrophoresis can be caused by factors such as improper loading of samples, insufficient DNA concentration, or issues with the gel or electrophoresis equipment.


What has the author A J Houtsmuller written?

A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins


What do the bands in gel electrophoresis represent?

The bands in gel electrophoresis represent different sizes of DNA fragments.


What kind of macromolecules can you look at with gel electrophoresis do you need to use different techniques for the different kinds of macromolecules?

You can look at nucleic acids (DNA and RNA) and proteins using gel electrophoresis. However, different techniques are needed for each type of macromolecule. For nucleic acids, agarose gel electrophoresis is commonly used, while for proteins, polyacrylamide gel electrophoresis is typically employed.