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What oligonucleotide probe would best hybridize with the DNA sequence 5'-AAAAGGTTCC-3'?
Wiki User
∙ 15y ago
3' TTTTCCAAGG 5' for a DNA probe; 3' UUUUCCAAGG 5'for an RNA probe.
The reasons:
1
Paired nucleic acid strands are antiparallel, meaning that they run in opposite directions. So the 5' end of one strand is opposite the 3' end of the other strand.
2
Hybridizing follows base pairing rules: A pairs with T (in DNA) or U (in RNA), and C pairs with G.
Wiki User
∙ 15y ago
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Which feature of DNA enables a single-stranded DNA probe to hybridize with a single-stranded target gene?
Complementary base
What is a blunt probe used for?
Blunt means "not sharp". Sometimes you want a knife or a needle, but not this time. probe (prōb) 1. a long, slender instrument for exploring wounds or body cavities or passages. 2. a radioactive or chemiluminescent DNA or RNA sequence used to detect the presence of a complementary sequence. DNA probes are used clinically to detect and identify infectious disease agents.
Is a bio probe the same as a bio sensor?
While a bioprobe has some things in common with a biosensor, most biosensors are not bioprobes, and most bioprobes are not biosensors. A biosensor is a kind of sensor -- i.e., it is used to detect something -- that uses at least some biological tissue in its construction. Perhaps the most well-known biosensor is the miner's canary, a small bird in a cage used to detect carbon monoxide and other deadly gases. The thing sensed by a biosensor is not necessarily biological -- as in this case, carbon monoxide. A bioprobe is a kind of sensor used to detect some kind of biological thing. Perhaps the most well-known bioprobe is the blood pressure meter, used to measure blood pressure. The bioprobe itself -- in this case, the pressure cuff -- is not necessarily biological. A hybridization probe is used to detect a particular DNA sequence. Because the thing it is designed to detect (a particular DNA sequence) is biological, and the hybridization probe itself is made of something biological (the complementary DNA sequence), a hybridization probe is simultaneously a bioprobe and a biosensor.
Applications of chromosome walking and jumping?
You want to know the sequence of a gene and only know the sequence of 2 DNA markers near it such as an RFLP (stands for Restriction fragment length polymorph) or another gene. Using different restriction enzymes, cut the genome of the organism to create DNA clones which overlap. For example, if the genome is ABCDEFGHI, enzyme 1 cuts it into 2 pieces to make ABCD EFGHI and enzyme 2 cuts it into 3 pieces to make ABC DEF GHI.. These pieces will over lap in sequence because the enzymes have different restriction sites. Using the known sequence of the RFLP, a radio labeled nucleic acid probe can be made to search the genomic library. The probe will anneal to clones with overlaps, and the ends of these clones can be made into new probes to search for the adjacent sequence. This is repeated until the desired segment has been cloned. each clone is sequenced and the overlaps are compared to determine the order of the sequences. This technique can be used to analysis diseases, such as cystic fibrosis, to look for mutations.
What are the functions of a dissecting probe?
Dissecting Probes & Dissecting Needlesnobody has any idea
If the partial sequence A of rRNA to be detected in environmental engineering system is shown 5'-cggguuagcgcaccgc-3' Design the oligonucleotide probe for the detection of the sequence A only?
8
Explain why a probe will hybridize to a target gene but not to any unrelated genes?
We design probes to be specific so that they will hybridize only to the target gene but not to random, unrelated genes. A probe hybridizes to a sequences to which it is complementary to. Consequently, the longer the probe is, the more specific it will be.
Why does a probe hybridize to a target gene but not to any other unrelated gene?
Complementary base pairing occurs only between the probe and the target gene.
Which feature of DNA enables a single-stranded DNA probe to hybridize with a single-stranded target gene?
Complementary base
What explains why a probe will hybridized to a target gene but not to any unrelated genes?
We design probes to be specific so that they will hybridize only to the target gene but not to random, unrelated genes. A probe hybridizes to a sequences to which it is complementary to. Consequently, the longer the probe is, the more specific it will be.
What is a cDNA probe?
These are the DNA probes (searching tool for DNA sequences) are made up of short DNA fragment and lablled with Biotin. The biotin bound to dUTP generally at the 11th position, eg.. biotin-11-dUTP. These lablled neucleotide are incorportaed using the procedure of polymerase chain reaciton (PCR) as the labeling is carried out using a doped nucleotide mix at ratios from 1 to 10% of the labeled nucleiotide to its unlabeled counterpart. The biotinylated lablled probe are used in southern blotting , plaque lift, colony lift, in situ hybridization.. The enzyme coupling reaciton is Streptavidn affinity. Detecting methods are Colorimetric, chemiluminiscece, immunogold, and fluorescence
What is a blunt probe used for?
Blunt means "not sharp". Sometimes you want a knife or a needle, but not this time. probe (prōb) 1. a long, slender instrument for exploring wounds or body cavities or passages. 2. a radioactive or chemiluminescent DNA or RNA sequence used to detect the presence of a complementary sequence. DNA probes are used clinically to detect and identify infectious disease agents.
What is the probe size?
Probe,probe se 2.0 / probe gt 2.5
What is space probe?
Space probe is a station.
What is the brake bleeding sequence on an 1989 Ford Probe GT?
pass rear,driver rear,pass front,driver front Rear right, rear left, front right, front left.
What are Probe owners clubs?
I would think a Probe owners club is for owners of Ford Probe cars.
How does recombinant DNA technology identify the position of a gene on a chromosome?
A probe may be used, a probe is a specific sequence of DNA tracked by using fluorescent dye. This probe, whose base pairs are known, can be added to another set of DNA. where this probe attaches (seen by adding fluorescent dye to probe), the DNA is seen to be complimentary. These probes have the ability to bond to single complementary strands of DNA from samples. This can be used to determine which chromosome and specific position on the chromosome is complimentary to the known probe DNA. this technique is important to identify abnormal gene sequences, by using thes abnormal probes to test for such genes which may be detrimental to human health. see: http://www.hsc.csu.edu.au/biology/options/genetics/3060/genetics_974.html
