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What oligonucleotide probe would best hybridize with the DNA sequence 5'-AAAAGGTTCC-3'?
3' TTTTCCAAGG 5' for a DNA probe; 3' UUUUCCAAGG 5'for an RNA probe.
The reasons:
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Paired nucleic acid strands are antiparallel, meaning that they run in opposite directions. So the 5' end of one strand is opposite the 3' end of the other strand.
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Hybridizing follows base pairing rules: A pairs with T (in DNA) or U (in RNA), and C pairs with G.
What else can I help you with?
Which feature of DNA enables a single-stranded DNA probe to hybridize with a single-stranded target gene?
The complementary base pairing between adenine (A) and thymine (T), and cytosine (C) and guanine (G) allows a single-stranded DNA probe to hybridize with a single-stranded target gene. This specificity is key for detecting and identifying specific sequences in the target gene through base pairing interactions.
Which of the following determines the specificity of a DNA probe?
complementary base pairing-apex
How can one effectively design an in situ hybridization probe for accurate and specific detection of target sequences?
To design an effective in situ hybridization probe for accurate and specific detection of target sequences, one should consider the following steps: Select a target sequence that is unique to the gene of interest. Design a probe that is complementary to the target sequence and is of appropriate length (usually around 20-30 base pairs). Avoid regions of high sequence similarity with other genes to prevent non-specific binding. Consider the melting temperature (Tm) of the probe to ensure optimal hybridization conditions. Label the probe with a detectable marker, such as a fluorescent dye or enzyme, for visualization. Test the probe for specificity and sensitivity using control samples before conducting the in situ hybridization experiment.
A Probe is used in which stage of the gene transfer process?
A Probe is used in the detection stage of the gene transfer process. It is a short, single-stranded DNA fragment that can hybridize to complementary DNA sequences and help identify if a specific gene has been successfully transferred into a host cell. It is often used in techniques such as Southern blotting or fluorescence in situ hybridization (FISH).
What property of DNA makes hybridization between a labeled probe and a target gene possible?
The complementary base pairing between DNA strands enables hybridization between a labeled probe and a target gene. The hydrogen bonding between adenine-thymine and guanine-cytosine base pairs allows the probe to specifically bind to its complementary sequence in the target gene, facilitating detection.
If the partial sequence A of rRNA to be detected in environmental engineering system is shown 5'-cggguuagcgcaccgc-3' Design the oligonucleotide probe for the detection of the sequence A only?
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Why a probe will hybridize to a target gene?
A probe will hybridize to a target gene due to complementary base pairing between the nucleotides of the probe and the target sequence. This specificity allows the probe, often labeled for detection, to bind to its complementary region on the target gene under appropriate conditions, such as temperature and salt concentration. The hybridization process is driven by the stability of the double-stranded DNA formed, which is influenced by factors like sequence complementarity and the presence of chemical modifications. This property is widely utilized in techniques such as PCR, microarray analysis, and in situ hybridization for gene detection and analysis.
Explain why a probe will hybridize to a target gene but not to any unrelated genes?
We design probes to be specific so that they will hybridize only to the target gene but not to random, unrelated genes. A probe hybridizes to a sequences to which it is complementary to. Consequently, the longer the probe is, the more specific it will be.
Why does a probe hybridize to a target gene but not to any other unrelated gene?
Complementary base pairing occurs only between the probe and the target gene.
What explains why a probe will hybridized to a target gene but not to any unrelated genes?
We design probes to be specific so that they will hybridize only to the target gene but not to random, unrelated genes. A probe hybridizes to a sequences to which it is complementary to. Consequently, the longer the probe is, the more specific it will be.
Which feature of DNA enables a single-stranded DNA probe to hybridize with a single-stranded target gene?
The complementary base pairing between adenine (A) and thymine (T), and cytosine (C) and guanine (G) allows a single-stranded DNA probe to hybridize with a single-stranded target gene. This specificity is key for detecting and identifying specific sequences in the target gene through base pairing interactions.
Which of the following determines the specificity of a DNA probe?
complementary base pairing-apex
Why is the hybridization probe said to be complementary?
The hybridization probe is said to be complementary because it is designed to bind specifically to its complementary target sequence in the DNA or RNA of interest. This complementary binding is necessary for the probe to accurately detect and identify the target sequence in a sample.
What is a sense probe in insitu hybridisation?
A sense probe in in situ hybridization is a nucleotide sequence that is complementary to the target RNA or DNA sequence of interest, allowing for the detection of specific genes within tissue samples. Unlike antisense probes, which bind to the target sequence to visualize gene expression, sense probes bind to the non-template strand and serve as a control to confirm the specificity of the hybridization signal. The presence of a signal with a sense probe typically indicates non-specific binding, while a lack of signal supports the specificity of the antisense probe results.
How can one effectively design an in situ hybridization probe for accurate and specific detection of target sequences?
To design an effective in situ hybridization probe for accurate and specific detection of target sequences, one should consider the following steps: Select a target sequence that is unique to the gene of interest. Design a probe that is complementary to the target sequence and is of appropriate length (usually around 20-30 base pairs). Avoid regions of high sequence similarity with other genes to prevent non-specific binding. Consider the melting temperature (Tm) of the probe to ensure optimal hybridization conditions. Label the probe with a detectable marker, such as a fluorescent dye or enzyme, for visualization. Test the probe for specificity and sensitivity using control samples before conducting the in situ hybridization experiment.
How can a probe find a single-stranded target DNA gene apex?
A probe can find a single-stranded target DNA gene apex by hybridizing to its complementary sequence. This is often achieved through techniques like fluorescence in situ hybridization (FISH) or polymerase chain reaction (PCR), where the probe is labeled with a fluorescent tag or a reporter molecule. The specificity of the probe ensures that it binds only to the target sequence, allowing researchers to detect and analyze the gene of interest among other genetic material.
What allows a DNA probe to find a single-stranded target gene?
Diploid cells
