DNA ligase
DNA Ligase
1. Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene? Answer: BamHI, HaeIII, and HindIII 2. Which enzymes(s) would cut the plasmid without disrupting the function of the amp^R gene? Answer: BamHI, EcoRI, and HaeIII 3. Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid? Answer: BamHI, EcoRI, and HindIII 4. Which one restriction enzyme satisfies all three of the requirements listed above? Answer: BamHI only
It contains a gene for luciferase, a Lux gene (the enzyme that catalyzes the light-emitting reaction) and genes for enzymes which produce the luciferins (which are the substrates for the light-emitting reaction.). This causes bacterial cells to glow!
functions as a vector
i am from russia. every russian scientist knows that the gene that codes for an enzyme needed by the proteins is mitochondrion and ribosomes.
enzyme
DNA ligase
chips
If you are trying to take a gene from a DNA strand and put insert it into a plasmid, you wouldn't want a restriction enzyme to cut that gene up, or else it would be pretty useless. In other words, you need an enzyme or two that cuts outside that gene so that it can be functional after it's inserted into a plasmid. After your gene of interest is inserted into a plasmid, the plasmid can be put back into a bacterium, then you could genetically engineer plants with it or let the bacterium reproduce and produce many copies of a protein that you had wanted to make in the first place.
1. Scientists remove plasmids, small rings of DNA, from bacterial cells. 2. An enzyme cuts open the plasmid DNA. The same enzyme removes the human insulin gene from its chromosome. 3. The human insulin gene attaches the open ends of the plasmid to form a closed ring. 4. Some bacterial cells take up the plasmids that have the insulin gene. 5. When cells reproduce, the news cells will contain copies of the engineered plasmid. The foreign gene directs the cell to produce human insulin.
If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
Extract DNA from the cells of people who can make the digestion enzyme. Cut the DNA with restriction enzymes to cut out the gene that codes for the enzyme. Use gel electrophoresis to locate the gene. Then, use polymerase chain reaction to make copies of the gene. Choose a plasmid that has an antibiotic-resistance genetic marker, and cut the plasmid with the smae restriction enzyme use to cut out the hyman gene. Insert the copies of the human gene into the plasmids. Allow bacterial cells to take in the plasmids. Select for transformed bacteria by growing them in a culture containing the antibiotic. These bacteria will make the digestion enzyme.
The pGlo plasmid contains an ampicillin resistance gene. This gene encodes an enzyme, β lactimase, which enzymatically degrades ampicillin. Therefore, bacteria that take up the plasmid (transformants) become resistant to ampicillin.
1. Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene? Answer: BamHI, HaeIII, and HindIII 2. Which enzymes(s) would cut the plasmid without disrupting the function of the amp^R gene? Answer: BamHI, EcoRI, and HaeIII 3. Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid? Answer: BamHI, EcoRI, and HindIII 4. Which one restriction enzyme satisfies all three of the requirements listed above? Answer: BamHI only
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It contains a gene for luciferase, a Lux gene (the enzyme that catalyzes the light-emitting reaction) and genes for enzymes which produce the luciferins (which are the substrates for the light-emitting reaction.). This causes bacterial cells to glow!