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In prokaryotes: DNA gyrase - a topoisomerase II protein that introduces negative supercoils in the DNA to reduce torsional stress
DNA helicase - breaks the hydrogen bonds between nucleotides and unzips the DNA double helix
Single stranded binding proteins - stabilize DNA be keeping the DNA strands from reannealing
DNA Polymerase III - polymerizes nucleotides forming a new DNA strand
Loading clamp - loads the DNA strand into pol III
DNA Primase - lays down an RNA primer on the lagging strand
DNA Polymerase I - replaces RNA primer with DNA
DNA Ligase - ligates DNA strand together on the lagging strand
In eukaryotes:
DNA Pol III is replaced by DNA Pol Delta
Pol I is replaced by Pol Alpha
RPA - replication protein A replaces the SSBs
RFC - replication factor C replaces DNA loading clamp
PCNA replaces the beta subunit
Wiki User
∙ 14y ago
Wiki User
∙ 9y agoTopoisomerase is a key player in replication. The enzyme helps to relieve the twisting of DNA molecules that have resulted from helicase.
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What is Major enzyme involved in replication?
DNA polymerase
Which enzyme unwinds DNA to initiate replication?
Helicase
Why DNA replication is popularly known as enzyme dependent?
DNA replication is aided by enzymes. Without the enzymes DNA will not be able to replicate.There are three main enzymes involved-Helicase - This enzyme separates the two parental DNADNA Polymerase - This enzyme exists in different forms and each one of them have a specific function in the replication of DNA.In short, it enhances each strands, adds base pairs and repairs any damage done to the strands during the replication process.Ligase - This enzyme puts the two stands together after the replication is complete.
Which is the main enzyme used for the replication of DNA in ecoli?
DNA polymerase
What molecule unwinds DNA?
A replication bubble.
What is the name of the enzyme that aids in the process of replication?
The enzyme DNA dependent DNA polymerase is essential for DNA replication
What does the helicase enzyme do?
Helicase is an enzyme involved in DNA replication. It unwinds and unzips the parental DNA strand.
What enzyme plays a role in replication?
the make food
Major enzyme involved in replication?
RNA polymerase
What is Major enzyme involved in replication?
DNA polymerase
Which enzyme unwinds DNA to initiate replication?
Helicase
Compare the polymerase chain reaction and DNA replication?
PCR need thermostable enzyme like taq DNA polymearse, while in replication using highly proofreading enzyme DNA polymerase. taq enzyme work in very high temprature while in replication our body temprature
What is the enzyme that untwists the DNA molecule before replication?
helicase
What enzyme is involved in DNA replication?
Helicase and DNA polymerase
Why DNA replication is popularly known as enzyme dependent?
DNA replication is aided by enzymes. Without the enzymes DNA will not be able to replicate.There are three main enzymes involved-Helicase - This enzyme separates the two parental DNADNA Polymerase - This enzyme exists in different forms and each one of them have a specific function in the replication of DNA.In short, it enhances each strands, adds base pairs and repairs any damage done to the strands during the replication process.Ligase - This enzyme puts the two stands together after the replication is complete.
Y shaped regions where the 2 strands of DNA separate?
It is called a replication fork.
What enzyme replicates DNA during semi-conservative replication?
Polymerase
