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Two major enzymes used during DNA replication?
Two major enzymes used during DNA replication are DNA polymerase, which synthesizes new DNA strands by adding nucleotides in a complementary manner, and DNA helicase, which unwinds the DNA double helix to expose the template strands for replication.
Does RNA polymerase require a primer for transcription?
No, RNA polymerase does not require a primer for transcription.
Which of the following removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments?
DNA polymerase I removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments in prokaryotes.
What enzyme Cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides?
The enzyme that cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides is DNA polymerase I in prokaryotes and DNA polymerase delta in eukaryotes. This process, known as primer removal or primer excision, is essential for completing DNA replication accurately.
Why doesn't RNA polymerase require a primer for transcription?
RNA polymerase does not require a primer for transcription because it can initiate the process on its own by recognizing specific DNA sequences called promoters. This allows RNA polymerase to bind to the DNA and start synthesizing RNA without the need for a primer like DNA polymerase does during DNA replication.
Two major enzymes used during DNA replication?
Two major enzymes used during DNA replication are DNA polymerase, which synthesizes new DNA strands by adding nucleotides in a complementary manner, and DNA helicase, which unwinds the DNA double helix to expose the template strands for replication.
Does RNA polymerase require a primer for transcription?
No, RNA polymerase does not require a primer for transcription.
What is the function of primer in PCR?
it synthesizes a single RNA primer at the 5' end of the leading end.
What is a RNA primer removed by?
A RNA primer in DNA replication is removed by an enzyme called DNA polymerase I in prokaryotes and DNA polymerase δ in eukaryotes. These enzymes have exonuclease activity that can remove RNA primers and replace them with DNA nucleotides.
What does DNA primase create?
DNA primase creates RNA primer. DNA primase is an enzyme and DNA polymerase uses the RNA primer to replicate ssDNA.
Which of the following removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments?
DNA polymerase I removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments in prokaryotes.
What does the first nucleotide attach to because DNA polymerase can only add nucleotides to a pre-existing strand?
A primer molecule is required for DNA polymerase to initiate the addition of nucleotides. This primer provides a starting point for DNA polymerase to begin adding nucleotides in the correct sequence. Once the primer is in place, DNA polymerase can add nucleotides complementary to the template strand.
What enzyme Cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides?
The enzyme that cuts out the RNA primer on the replicated DNA molecule and replaces it with the appropriate DNA nucleotides is DNA polymerase I in prokaryotes and DNA polymerase delta in eukaryotes. This process, known as primer removal or primer excision, is essential for completing DNA replication accurately.
Why doesn't RNA polymerase require a primer for transcription?
RNA polymerase does not require a primer for transcription because it can initiate the process on its own by recognizing specific DNA sequences called promoters. This allows RNA polymerase to bind to the DNA and start synthesizing RNA without the need for a primer like DNA polymerase does during DNA replication.
In polymerase chain reaction how many kinds of primer are used?
In polymerase chain reaction (PCR), two types of primers are used: a forward primer and a reverse primer. These short DNA sequences are specific to the target DNA region to be amplified and serve as starting points for DNA synthesis by the DNA polymerase enzyme.
Why does DNA polymerase require a primer for initiating the synthesis of new DNA strands?
DNA polymerase requires a primer to initiate the synthesis of new DNA strands because it can only add nucleotides onto an existing strand of DNA. The primer provides a starting point for the polymerase to begin adding nucleotides and building the new DNA strand.
Which of the following is needed in the process of DNA replication?
The ingredients needed for DNA replication include a short oligonucleotide primer and dNTPS. It also needs DNA polymerase and different transcription and translation factors.
