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Because it is a type of stain that uses contrasting (in this case the presence or absence of a stain) color to determine what type or classification the organism is. Differential = determining between different groups; you are staining to differentiate one group from another by classifying the organisms' ability to quickly resist decolorization by acids.
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∙ 13y ago
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∙ 15y agoFirst - The purpose of staining (in general) is to enable one to see cells microscopically by adding color to the cell. This is due to the fact that unstained bacteria are transparent and are difficult to observe. Acid fast stain is a differential staining procedure (uses more than one stain). This stain is used to distinquish species of MYCOBACTERIUM (the causitive agent of tuberculosis and Leprosy).
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∙ 12y agoBecause in a culture of bacterium, it stains the bacteria that are pathogenic (disease causing).
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∙ 15y agoFast Stain Acid Is Uselss, check out this great band. www.theused.net
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∙ 13y agoAcid fast stains are used to stain microbacters (such as tuberculosis) that possess a thick, waxy cell wall that isn't completely stained by a normal staining process.
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Is mordant used in acid fast stain?
The heat is the mordant for an acid-fast stain
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Is a Gram stain an adequate substitute for an acid fast stain?
So few organisms are acid-fast, the acid fast stain is used only when infection by an acid-fast organisms is suspected.
Is the mycobacterium smegmatis positive to acid-fast?
NO, s. epidermidis does not show up positive in an acid fast stain. The acid fast stain is a differential stain that differentiates cells with mycolic acids in their cells walls with those who do not. cells with mycolic acid are therefore acid fast. s epidermidis does not contain this waxy substance in their cell wall so it doesnt fall into this category.
Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?
Safranin
Are endospores acid fast?
No, endospores are not acid fast because they are not cells. Endospores will stain with the acid fast stain and look fuchsia but they are not positive for acid fast.
What is the decolorizing agent in the acid fast stain?
The decolorizing agent in the acid fast stain is acid alcohol. The decolorizing agent in the gram stain is ethanol.
What are the name of cold acid fast stain?
modified kinyoun's acid fast stain (cold)
Is mordant used in acid fast stain?
The heat is the mordant for an acid-fast stain
Can use carbolfuchsin as a counterstain?
The carbolfuchsin is a differential stain. It is an acid-fast stain which distinguishes organisms with waxy cell walls with the use of acid alcohol.
What color is Brilliant green K in Acid fast cell?
Acid-fast bacteria stain pink. Non acid-fast bacteria stain green.
What are the advantages and disadvantages of acid fast stain?
Acid Fast Stains Suck Anus
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Is a Gram stain an adequate substitute for an acid fast stain?
So few organisms are acid-fast, the acid fast stain is used only when infection by an acid-fast organisms is suspected.
Which color is common to both the gram stain and the acid-fast stain?
purple
Is the mycobacterium smegmatis positive to acid-fast?
NO, s. epidermidis does not show up positive in an acid fast stain. The acid fast stain is a differential stain that differentiates cells with mycolic acids in their cells walls with those who do not. cells with mycolic acid are therefore acid fast. s epidermidis does not contain this waxy substance in their cell wall so it doesnt fall into this category.
Why harsh treatment is necessary in acid-fast stain?
becausee..
