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Why do you use an inoculating loop instead of a needle to make the transfer from the culture plates to the culture tubes?
Because the solid media is more dense over a smaller area so a inoculating needle is used to retrieve the specimen.
Where as for a liquid medium the specimen is more spread out over the liquid. The inoculating loop can collect more liquid because there is more metal present at the inoculating specimen retrieval point and has the ability to collect liquid in the loop.
I'm currently taking general microbiology and my lab book hardly covers this.
A.C.
What else can I help you with?
Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
In subculturing when do you use the inoculating loop?
Se utiliza una vez el asa de siembra o la aguja se encuentra visiblemente roja, de manera que mueran todos los organismos. De esta manera poder esparcir a su vez la muestra en la laminilla.Translation: It is used once the loop or needle is visibly red, so that all organisms die. Thus turn to spread the sample on the slide.
What is agar stab?
An agar stab is a microbiological technique used to maintain and preserve bacterial cultures. A sterile inoculating needle is used to introduce bacteria into a solid agar medium by stabbing it vertically into the tube. This method allows for long-term storage of bacterial cultures and helps to maintain their viability.
Why should use an inoculating needle when making smears from a solid media?
You should use an inoculating needle when making smears from solid media because of the solid's density. The smaller areas are denser, so it is easier to retrieve these specimens using an inoculating needle.
What are the other inoculating instruments aside from needle?
Some other inoculating instruments include loops, spreaders, swabs, and pipettes. These tools are used to transfer and spread microorganisms onto various growth media for cultivation and observation in laboratory settings.
Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
What is an inoculating needle used for?
An inoculating needle is used in a microbiology lab to collect microorganisms from a culture and move them to a petri dish.
What is an inoculation?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
How inoculating needle use?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
What is the function of needle in microbiology?
In microbiology, a needle, often referred to as an inoculating needle, is used to transfer microorganisms from one culture medium to another, particularly in techniques like streak plating or inoculating agar slants. It allows for precise manipulation of small volumes of liquid or solid cultures, ensuring that specific microbial strains can be isolated and studied. The needle's design facilitates sterile handling and minimizes contamination, which is crucial for accurate experimentation and analysis.
Inoculating an agar deep tube?
inoculation needle
Why must a straight inoculating needle be used when inoculating an agar deep tube?
This is important to prevent the inoculating needle from becoming stuck in the agar, taking out pieces of agar while trying to remove the instrument. This agar will get into the inoculum when sterilizing the needle on the flame, causing contamination to your sample.
What are other inoculating instruments aside from needle?
Air gun and inoculating loop are some of the another inoculating instruments. The air gun is used to squirt thin stream of vaccine.
In subculturing when do you use the inoculating loop?
Se utiliza una vez el asa de siembra o la aguja se encuentra visiblemente roja, de manera que mueran todos los organismos. De esta manera poder esparcir a su vez la muestra en la laminilla.Translation: It is used once the loop or needle is visibly red, so that all organisms die. Thus turn to spread the sample on the slide.
What is agar stab?
An agar stab is a microbiological technique used to maintain and preserve bacterial cultures. A sterile inoculating needle is used to introduce bacteria into a solid agar medium by stabbing it vertically into the tube. This method allows for long-term storage of bacterial cultures and helps to maintain their viability.
Why do you use an inoculating loop to transfer fromt he culture plates to the culture tubes?
You can transfer more because of the adhesive interactions of the water create that drop in the loop and you can spread it more easily. Also a needle could pierce the gel you transfer to because of its pointy end. The loop on the other hand distributes its force over a greater area so it does not damage the gel. This is a good answer if going from the tube to the plate, but backwards for the actual question. You use a loop to pick up a well isolated colony from your streak plate to introduce into the culture. You also can use a needle, but you won't get as many cells. However, in my work I just use sterilized toothpicks.
Why should use an inoculating needle when making smears from a solid media?
You should use an inoculating needle when making smears from solid media because of the solid's density. The smaller areas are denser, so it is easier to retrieve these specimens using an inoculating needle.
