It may be necessary to continually focus a microscope so as to get accurate results about a specimen. This will give you enough time to study any specimen.
continually adjusting the focus of a microscope helps us maintain the good eyesight we have and keeps us from wearing graded glasses .
The objective on a microscope is comprised of a tube and one or more lenses, and may include a mirror as well. Its purpose is to gather and focus light, typically while magnifying the image, from the sample or object under study.
There're two knobs at the side of a light microscope (this is just my experience, it may be different if you're using different brands of microscope or even an electron type microscope). For first focus, adjust the larger knob (also known as rough adjustment) followed by the final focus (to sharpen the image), by adjusting the smaller knob (known as fine adjustment).
Microscope is the basic tool. Some other equipments may be needed for the preparation.
When using a microscope, the initial focusing of any slide is done under either the 4x or 10x objective. Once you have your subject in focus, you may make further adjustments.
1. Ocular Lens (Eyepiece) - where you look through to see the image of your specimen. Magnifies the specimen 10X actual size. 2. Body tube - the long tube that supports the eyepiece and connects it to the objectives. 3. Nosepiece - the rotating part of the microscope at the bottom of the body tube; it holds the objectives. 4. Objective Lenses - (low, medium, high). Depending on the microscope, you may have 2, 3 or more objectives attached to the nosepiece; they vary in length (the shortest is the lowest power or magnification; the longest is the highest power or magnification). 5. Arm - part of the microscope that you carry the microscope with; connects the head and base of the microscope. 6. Coarse Adjustment Knob - large, round knob on the side of the microscope used for "rough" focusing of the specimen; it may move either the stage or the upper part of the microscope. Location may vary depending on microscope - it may be on the bottom of the arm or on the top. 7. Fine Adjustment Knob - small, round knob on the side of the microscope used to fine-tune the focus of your specimen after using the coarse adjustment knob. As with the Coarse Adjustment Knob, location may vary depending on the microscope. 8. Stage - large, flat area under the objectives; it has a hole in it (see aperture) that allows light through; the specimen/slide is placed on the stage for viewing. 9. Stage Clips - clips on top of the stage which hold the slide in place. 10. Aperture - the hole in the stage that concentrates light through the specimen for better viewing. 11. Diaphragm - controls the amount of light going through the aperture; may be adjusted. 12. Light or Mirror - source of light usually found near the base of the microscope; used to direct light upward through the microscope. The light source makes the specimen easier to see.
because the subject may have different charactreistics at different depths
How may humans have you seen drinking continually? If it were necessary, we would have graduated from using our mouths to having a drip inserted in to us. Also, do some research on water intoxication.
The object may be 3D. If it is, not everything inside the object will be focussed. For example, if you look at a plant cell, the focus must be changed to look at the different organelles.
The objective on a microscope is comprised of a tube and one or more lenses, and may include a mirror as well. Its purpose is to gather and focus light, typically while magnifying the image, from the sample or object under study.
The objective on a microscope is comprised of a tube and one or more lenses, and may include a mirror as well. Its purpose is to gather and focus light, typically while magnifying the image, from the sample or object under study.
The objective on a microscope is comprised of a tube and one or more lenses, and may include a mirror as well. Its purpose is to gather and focus light, typically while magnifying the image, from the sample or object under study.
The objective on a microscope is comprised of a tube and one or more lenses, and may include a mirror as well. Its purpose is to gather and focus light, typically while magnifying the image, from the sample or object under study.
The objective on a microscope is comprised of a tube and one or more lenses, and may include a mirror as well. Its purpose is to gather and focus light, typically while magnifying the image, from the sample or object under study.
The objective on a microscope is comprised of a tube and one or more lenses, and may include a mirror as well. Its purpose is to gather and focus light, typically while magnifying the image, from the sample or object under study.
Optical, though to see smaller organelles and structures in higher resolution an electron microscope may be necessary.
stereoscopic microscope is the most frequently used and versatile microscope found in a crime lab. Its wide field of view and great depth of focus make it ideal for locating trace evidence that may be found at the crime scene. Furthermore its potentially large working distance makes it quite applicable for the microscope examination of big bulky objects.
At a higher power the field of view is smaller and the object may no longer be in the field of view. Or it may have gone out of focus.