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If native DNA is isolated carefully the sample will consist of long fragments of double stranded DNA. The hydrodynamic behaviour of this molecule resemble a long rod which because is highly asymetric it has a high intrinsic viscosity. As DNA denatures the two strands separate and each single strand is free to adopt different conformations in a form of a random coil. This form has a lower intrinsic viscosity than the double stranded DNA.
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Why do you keep competent cells in ice?
to avoid denaturation of the genetic material in this case DNA
Why does DNA content decrease during mitosis?
During mitosis, DNA content in the cell decreases. This is due to the fact that the cell is splitting in half, and it loses quite a bit of it's genetic material during the separation.
Which DNA nucleotide pair is most stable?
GC pairing is most stable and require maximum energy to dissoicate. This is reason that rate of DNA denaturation depends upon the GC content of DNA.
Why heat DNA to 94 degrees?
depends what you intend to do, usually in a pcr reaction it denatures the DNA so you get 2 seperated strands
What happens to a population when genetic variation decreases?
Less sex mutation and changes in DNA, I think?
Effect of hcl on DNA?
denaturation
How does high salt concentration influence denaturation kinetics of DNA?
High salt concentration slows the denaturation of DNA because it stabilizes the charges
Why initial denaturation step is done before denaturation step in PCR?
To make sure the double-strand DNA template is separated into single strands.
How do you destroy the hydrogen bonds holding DNA together?
It can be done by heating and the process is called denaturation of protein of DNA
Why extraction buffer is added for isolation of DNA?
Extraction Buffer is used to maintain pH of the solution.which prevents denaturation of DNA.
Why do you keep competent cells in ice?
to avoid denaturation of the genetic material in this case DNA
If e. coli DNA polymerase was used instead of thermus aquaticus DNA polymerase in a pcr polymerase chain reaction procedure what would happen?
Unlike Taq DNA polymerase, E.coli DNA polymerase is not heat-stable and will denature during the strand denaturation step of the PCR reaction.
Why does DNA content decrease during mitosis?
During mitosis, DNA content in the cell decreases. This is due to the fact that the cell is splitting in half, and it loses quite a bit of it's genetic material during the separation.
What is the mechanism of alkaline denaturation of dna?
Adding hydroxyl ions will deprive protons from DNA molecules that are needed to form a hydrogen bridge bond.
Why you give denaturation and neutralization to gel treatment?
For denaturation :-To eliminate hydrogen bonds with sodium hydroxide (NaOH)To denature double stranded DNA into single stranded DNAFor neutralization :-Neutralize the gel to get the pH that DNA can bind to the membrane.Destroy any remaining RNA present in sample
Which DNA nucleotide pair is most stable?
GC pairing is most stable and require maximum energy to dissoicate. This is reason that rate of DNA denaturation depends upon the GC content of DNA.
Why heat DNA to 94 degrees?
depends what you intend to do, usually in a pcr reaction it denatures the DNA so you get 2 seperated strands
