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The biuret reaction is based on the reduction of Cu2+ to Cu+ which then complexes with the nitrogen atoms on the peptide bonds. This reactionis carried out at high pH and gives a violet colour. It is the amide bond (common to all proteins) which is resposible for the colour.

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Does phenol give a positive biuret test?

No, phenol does not give a positive biuret test. The biuret test is used to detect the presence of proteins, not phenol. Phenol is a type of organic compound that does not contain the peptide bonds that the biuret test reacts with.


Does pepsin test positive in biuret test?

Pepsin does not test positive in the biuret test. The biuret test is used to detect the presence of proteins in a solution, which contain peptide bonds. Pepsin is an enzyme that breaks down proteins into smaller peptides, so it does not give a positive result in the biuret test.


Will glutamic acid give a positive biuret test?

Amino acids doesn't react with the biuret reagent.


How many of these chemical groupings must be present in a molecule to give a positive biuret test?

At least two peptide bonds must be present in a molecule to give a positive biuret test. The biuret reagent interacts with peptide bonds in proteins, forming a colored complex that can be detected visually.


How many chemical grouping must be present in a molecule to give a positive Biuret Test?

You must have at least two, those that will form the peptide linkage (NH2 and COOH) since Biuret test is for detecting peptide linkages.


Will lipids give positive result for biuret est?

No, lipids will not give a positive result for the Biuret test. The Biuret test is specifically designed to detect the presence of proteins, as it reacts with peptide bonds in amino acids. Lipids, which are primarily composed of fatty acids and glycerol, do not contain these peptide bonds and therefore do not react in this test.


Would albumin give a positive biuret test?

Hey. okay.. the biuret test is a test for peptide bonds. when CuSO4 is added to KOH, an alkaline solution, nitrogen atoms from the peptide bond forms a purple compound with the Cu2+ ions. hope this helps! read it in a text book.


Would glucose give a positive result with the Biuret test?

No, glucose would not give a positive result with the Biuret test. The Biuret test is specific for detecting proteins, not sugars like glucose. It works by reacting with peptide bonds in proteins to form a colored complex.


Will Alanine give a negative Biuret test?

Yes, alanine will give a negative Biuret test. The Biuret test is used to detect the presence of proteins, which are made up of long chains of amino acids. Since alanine is a single amino acid, it will not give a positive response in the Biuret test.


Is biuret test suitable to test the presence of proteins in the urine?

Yes, the biuret test will indicate the presence of peptides. If it turns violet, it is a positive result.


What colour is biuret before test?

Biuret solution is typically blue-violet in color before the test.


Which sample gives a negative Biuret test and why?

Gelatin and egg albumin.