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1mM DPPH (MW 394.32) solution would be 0.39 mg/1mL

1M = 390 mg/1mL

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How do you prepare 1 mM dpph radical solution?

To prepare a 1 mM DPPH radical solution, dissolve 3.94 mg of DPPH (2,2-diphenyl-1-picrylhydrazyl) in 1 liter of solvent (typically methanol or ethanol). This will result in a concentration of 1 mM (molecular weight of DPPH is approximately 394.3 g/mol).


How do you prepare dpph radical solution?

To prepare a DPPH radical solution, dissolve DPPH powder in a solvent like methanol or ethanol to a concentration of around 0.1-0.2 mM. Mix well until the solution is completely dissolved. The solution can be stored in the dark and at low temperature to protect it from light.


How do you prepare 0.2 milli molar DPPH solution in ethanol?

To prepare a 0.2 millimolar (mM) DPPH solution in ethanol, you need to dissolve 0.2 millimoles of DPPH in a final volume of 1 liter of ethanol. First, calculate the molecular weight of DPPH (usually around 394.33 g/mol) to determine the mass needed to dissolve in ethanol. Then add this mass of DPPH to ethanol and make up the volume to 1 liter.


How do you store a DPPH in methanol solution for long term purpose?

To store a DPPH in methanol solution for long-term purposes, it is recommended to seal the container tightly to prevent evaporation or contamination. Store the solution in a dark, cool place to minimize degradation of the DPPH. Periodically check the solution for any color changes or signs of degradation.


How do you prepare 300 mM dpph radical solution?

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What are the reagents used in the estimation of antioxidants?

Common reagents used in the estimation of antioxidants include DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent, Folin-Ciocalteu reagent, and Trolox standard. These reagents are utilized in assays such as DPPH radical scavenging assay, total phenolic content assay, and oxygen radical absorbance capacity (ORAC) assay to measure the antioxidant capacity of compounds.


How do you show the antioxidant properties of coriandrum sativum in a lively manner on a lab scale apart from using standard assays?

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