for prepare 1mM DPPH radical solution:
DPPH Molecular weight: C18H12N5O6 = 394g
DPPH 1mM = 394/1000= 0.394g/L
pl. suggest how to prepare 50 sulphuric acid
to prepare 100ml of 100mM Trissolution: Mol wt of Tris=121.14121.14g in 1000ml ----> 1M12.11g in 100ml -------->1M1M=1000mM121.1g---->1000mM12.11g ----------->100mM1.211g in 100ml and 100mM Tris
the mass of the EDTA used to prepare 1L oi solution is 18.612g. The formula mass is 372.24 g/mol. Therefore concentration of the 50x solution is: 18.612 g 1 mol ----------- x --------- = 0.050000 mol/L = 50.000 mM 1L 372.24 g
5 mM phosphate buffer (4.82 g/l monohydrate, monosodium phosphate, pH 6.5).
- Transfer 0,057 mL of 30 % ammonia solution, with the aid of a micropipette in a volumetric flask (volume 100 mL) - Add distilled (or demineralized) water up to the mark, at 20 0C - Put the stopper and stir.
I really dont know people
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pl. suggest how to prepare 50 sulphuric acid
A water solution containing 50 mM tris(hydroxymethyl)aminomethane and 150 mM sodium chloride has a pH of 7,6.
show solution convert 0.015 km to mm
Also 150 mM of sodium.
Dilute a measured volume of the 100 mM solution with 19 times its own volume of pure water to produce a 5mM solution.
Preparation of 100 mM H₂O₂ Measure out 10 mL of 30% hydrogen peroxide (H₂O₂). Dilute the 10 mL of 30% H₂O₂ with 90 mL of deionized water. The resulting solution is roughly 100 mM H₂O₂.
to prepare 100ml of 100mM Trissolution: Mol wt of Tris=121.14121.14g in 1000ml ----> 1M12.11g in 100ml -------->1M1M=1000mM121.1g---->1000mM12.11g ----------->100mM1.211g in 100ml and 100mM Tris
To prepare the buffer using solid form reagents, prepare a 0.1 M ammonium acetate solution by dissolving 7.7 g ammonium acetate in a 1000 ml water. Adjust 1 L of this solution to pH 4.5 by adding acetic acid (about 8 ml) and 5 ml of 1 M p-TSA (equivalent to 5 mM p-TSA).
Concentrated MSA is 1.481 g/mole MW = 98.11 g/mole 4.5 mL of concentrated MSA to total volume 2 L gives ~ 35 mM 4.5 mL Conc MSA x 1.481 g/mL / 96.11 g/mole x 1000 mM/mole / 2L = 35 mM
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