it has to be prepared using methanol or ethanol at the concentration we required.
To prepare a 1 mM DPPH radical solution, dissolve 3.94 mg of DPPH (2,2-diphenyl-1-picrylhydrazyl) in 1 liter of solvent (typically methanol or ethanol). This will result in a concentration of 1 mM (molecular weight of DPPH is approximately 394.3 g/mol).
To prepare a 0.1M DPPH (2,2-diphenyl-1-picrylhydrazyl) solution, you would weigh 3.83 grams of DPPH powder and dissolve it in 1 liter of solvent (typically ethanol or methanol). Make sure to wear appropriate personal protective equipment and handle the chemical in a well-ventilated area.
To prepare a 0.01N KBr solution, dissolve 0.74g of KBr in 1 liter of water. This will give you a solution with a molarity of 0.01N for KBr.
To prepare a 0.5M glutaraldehyde solution, you would need to dilute a concentrated glutaraldehyde stock solution with the appropriate volume of water or buffer solution. Calculate the volume of stock solution needed based on the desired final volume and concentration, then dilute with the solvent. Finally, mix the solution thoroughly to ensure uniform distribution. Remember to follow safety protocols when working with glutaraldehyde, as it is a hazardous chemical.
A solution of ammonia can be used to prepare ammoniumsulfate by reacting it with a solution of sulfuric acid to produce a solution of ammonium sulfate, which can be dried if desired to prepare solid ammonium sulfate.
To prepare a 1 mM DPPH radical solution, dissolve 3.94 mg of DPPH (2,2-diphenyl-1-picrylhydrazyl) in 1 liter of solvent (typically methanol or ethanol). This will result in a concentration of 1 mM (molecular weight of DPPH is approximately 394.3 g/mol).
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To prepare a 0.2 millimolar (mM) DPPH solution in ethanol, you need to dissolve 0.2 millimoles of DPPH in a final volume of 1 liter of ethanol. First, calculate the molecular weight of DPPH (usually around 394.33 g/mol) to determine the mass needed to dissolve in ethanol. Then add this mass of DPPH to ethanol and make up the volume to 1 liter.
To prepare a 0.1M DPPH (2,2-diphenyl-1-picrylhydrazyl) solution, you would weigh 3.83 grams of DPPH powder and dissolve it in 1 liter of solvent (typically ethanol or methanol). Make sure to wear appropriate personal protective equipment and handle the chemical in a well-ventilated area.
To store a DPPH in methanol solution for long-term purposes, it is recommended to seal the container tightly to prevent evaporation or contamination. Store the solution in a dark, cool place to minimize degradation of the DPPH. Periodically check the solution for any color changes or signs of degradation.
Common reagents used in the estimation of antioxidants include DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent, Folin-Ciocalteu reagent, and Trolox standard. These reagents are utilized in assays such as DPPH radical scavenging assay, total phenolic content assay, and oxygen radical absorbance capacity (ORAC) assay to measure the antioxidant capacity of compounds.
To prepare a 0.01N KBr solution, dissolve 0.74g of KBr in 1 liter of water. This will give you a solution with a molarity of 0.01N for KBr.
See the two Related Questions to the left for the answer.The first is how to prepare a solution starting with a solid substance (and dissolving it). The second question is how to prepare a solution by diluting another solution.
To prepare a buffer solution which may be acidic. Titrate ethanoic acid (weak acid) with sodium ethanoate(salt).
To prepare a 0.5M glutaraldehyde solution, you would need to dilute a concentrated glutaraldehyde stock solution with the appropriate volume of water or buffer solution. Calculate the volume of stock solution needed based on the desired final volume and concentration, then dilute with the solvent. Finally, mix the solution thoroughly to ensure uniform distribution. Remember to follow safety protocols when working with glutaraldehyde, as it is a hazardous chemical.
By applying the principle of win-win solution.
A solution of ammonia can be used to prepare ammoniumsulfate by reacting it with a solution of sulfuric acid to produce a solution of ammonium sulfate, which can be dried if desired to prepare solid ammonium sulfate.