0
1X PBS
8 g NaCl
0.2 g KCl
1.44 g Na2HPO4
0.24 g KH2PO4
in 800 ml of distilled H2O.
Adjust the pH to 7.4 with HCl. Add H2O to 1 liter. Sterilize by autoclave.
10X PBS
- Dissolve the following in 800ml distilled H2O.
- 80g of NaCl
- 2.0g of KCl
- 14.4g of Na2HPO4
- 2.4g of KH2PO4
- Adjust pH to 7.4.
- Adjust volume to 1L with additional distilled H2O.
- Sterilize by autoclaving.
Related:
Wiki User
∙ 14y ago
Add your answer:
Earn +20 pts
What is the molarity of 1x PBS buffer and how to prepare 20 mM PBS buffer?
Molarity of 1X PBS is 10mM. To prepare 20mM PBS take 16g of NaCl, 0.4 g KCl, 2.88 g Na2HPO4 0.48 g KH2PO4 in 800 ml of distilled H2O. Adjust the pH to 7.4 with HCl. Add H2O to 1 liter.
Why Calcium and magnesium free PBS?
What cell walls are you using? In mammalian cell culture the reason you would use Ca/Mg free PBS is to help remove them from the surface they are attached to. The adherens proteins that adherent cells use to stick to whatever they are stuck to requires Ca2+ and Mg2+. Therefore if you simply want to wash the cells you have to use a buffer WITH Ca2+ and Mg2+.
What is chemical formula of galena?
Galena's chemical name is - lead sulfide (PbS)
Is PbS a base or acid?
acid
What is the chemical formula of led sulphite?
PbS
What is the function of PBS buffer?
The main function of PBS (phosphate buffer saline) is to maintain the pH of the solution. by Himanshu Goel Jaypee University of Information Technology
The pH of 10x PBS buffer?
PBS pH usually ranges between 7.2 and 7.6.
What is the molarity of 1x PBS buffer and how to prepare 20 mM PBS buffer?
Molarity of 1X PBS is 10mM. To prepare 20mM PBS take 16g of NaCl, 0.4 g KCl, 2.88 g Na2HPO4 0.48 g KH2PO4 in 800 ml of distilled H2O. Adjust the pH to 7.4 with HCl. Add H2O to 1 liter.
Why use a phosphate buffer in the lab?
The PBS solution is necessary to check the stability of materials that will be used for biological purposes.
What is the function of Phosphate Buffered Saline?
PBS (phosphate buffer saline) is used to maintain osmolarity of cells i.e. maintaining an isotonic environment. it is used to maintain pH of proteins at which its native structure could be maintained.
What is the name of the theme song for Independent Lens on PBS and who is credited for composing and performing it?
Thisis an original composition commissioned for the series in 2002 by Musikvergnuegen.
10x PBS buffer?
10X PBS (0.1M PBS, pH 7.4): NaCl --------------------------------------------- 80 g KCl----------------------------------------------- 2g Na2HPO4 (anhydrous) ----------------------- 14.4 g KH2PO4 (anhydrous) ------------------------- 2.4g Distilled water ------------------------------- 1000 ml Mix to dissolve and adjust pH to 7.4 Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.
Who is Martha on PBS Kids?
Martha on PBS Kids is Martha on PBS Kids.
What is PBS Kids?
PBS Kids 900:000 AM PBS Kids Sprout (satallite and Verizon) 789:087 AM PBS 200:000 AM
When did PBS YOU end?
PBS YOU ended in 2006.
When was PBS YOU created?
PBS YOU was created in 1998.
Was Barney on PBS?
Yes and it was on PBS Kids.
