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The question is in poorly worded. I will assume the question is "why adjust the pH of Tris buffer with HCl and not Sodium Acetate?" I would assume the answer is - because sodium acetate is the conjugate base of a weak acid, and HCl is a strong acid. Also the salts you would be putting into the solution as a result would be different.
I think the question is actually, "The pH of Tris is adjusted with HCl, why isn't the pH of sodium acetate adjusted with HCl?". I'm not sure of the answer exactly, but I've always assumed its because if you adjust the pH with glacial acetic acid instead of HCl, you won't introduce chloride ions.
What else can I help you with?
What is the difference between tris tris cl and tris hcl?
Tris(hydroxymethyl)aminomethane (Tris) is a common buffer used in biochemistry, while Tris HCl is Tris buffer combined with hydrochloric acid to adjust the pH. Tris buffer is neutral (pH 7-9), while Tris HCl is acidic with a pH around 4.5-8.6.
What is the buffer capacity of Tris HCl?
The buffer capacity of Tris HCl depends on its concentration and the pH range of interest. Typically, Tris HCl has a good buffering capacity around its pKa value of approximately 8.1. At this pH, Tris HCl can resist changes in pH when small amounts of acid or base are added.
What is Tris HCl?
"Tris" is a chemical compound used as a buffer. The full name is tris(hydroxymethyl)aminomethane. Tris has the ability to absorb counter ions (+H and -OH) so as to help keep the solution that they are in at a stable pH level. When the pH of Tris is set using HCl (hydrochloric acid) the buffer is called Tris HCl.
What Volume of 5M HCl to make tris buffer at pH 7.5?
The formula weight is 121.5 --> this is equivalent to 1M with 121.5g tris in 1L dH20. For a 5M stock, use 5x as much tris in the same 1L dh20.607.5 g tris into 800ml dH2O - stirring - then pH to 7.5 with 6M HCl and QS to your final volume of 1L
How do you prepare 20 mM tris hcl buffer pH 7.4?
to prepare 100ml of 100mM Trissolution: Mol wt of Tris=121.14121.14g in 1000ml ----> 1M12.11g in 100ml -------->1M1M=1000mM121.1g---->1000mM12.11g ----------->100mM1.211g in 100ml and 100mM Tris
What is the difference between TAE buffer and TE buffer?
The main difference is in composition. In TE common Tris buffer is bring down to pH 8 with HCl and EDTA is involved but in TAE instead of Tris HCl in TE Tris-acetate buffer is used.
What is the difference between tris tris cl and tris hcl?
Tris(hydroxymethyl)aminomethane (Tris) is a common buffer used in biochemistry, while Tris HCl is Tris buffer combined with hydrochloric acid to adjust the pH. Tris buffer is neutral (pH 7-9), while Tris HCl is acidic with a pH around 4.5-8.6.
What is the buffer capacity of Tris HCl?
The buffer capacity of Tris HCl depends on its concentration and the pH range of interest. Typically, Tris HCl has a good buffering capacity around its pKa value of approximately 8.1. At this pH, Tris HCl can resist changes in pH when small amounts of acid or base are added.
What is Tris HCl?
"Tris" is a chemical compound used as a buffer. The full name is tris(hydroxymethyl)aminomethane. Tris has the ability to absorb counter ions (+H and -OH) so as to help keep the solution that they are in at a stable pH level. When the pH of Tris is set using HCl (hydrochloric acid) the buffer is called Tris HCl.
What Volume of 5M HCl to make tris buffer at pH 7.5?
The formula weight is 121.5 --> this is equivalent to 1M with 121.5g tris in 1L dH20. For a 5M stock, use 5x as much tris in the same 1L dh20.607.5 g tris into 800ml dH2O - stirring - then pH to 7.5 with 6M HCl and QS to your final volume of 1L
How do you prepare 20 mM tris hcl buffer pH 7.4?
to prepare 100ml of 100mM Trissolution: Mol wt of Tris=121.14121.14g in 1000ml ----> 1M12.11g in 100ml -------->1M1M=1000mM121.1g---->1000mM12.11g ----------->100mM1.211g in 100ml and 100mM Tris
What are the ingredients contained in TD buffer?
137 mM NaCl, 25 mM Tris-HCl [pH 7.4], 0.7 mM Na2HPO4, 5 mM KCl
What is the role of tris-hcl sodium bisulfite and sodium bicarbonate in genomic DNA isolation?
than podo
How do you make a 1 mol tris buffer?
To make a 1 mol tris buffer, you would need to dissolve 121.1 g of Tris (Tris(hydroxymethyl)aminomethane) in water and dilute to a final volume of 1 liter. Adjust the pH with a strong acid like HCl or a strong base like NaOH to reach the desired pH of the buffer.
Function of tris HCl in DNA isolation?
Tris HCl is used as a buffer in DNA isolation to maintain a stable pH level during the process. It helps to prevent pH fluctuations that can affect the integrity of the DNA molecule. Tris HCl also aids in the solubilization of proteins and DNA, ensuring efficient extraction of DNA from the sample.
What is the recommended lysis buffer recipe for proteins extraction?
A commonly used lysis buffer recipe for protein extraction includes components such as Tris-HCl, sodium chloride, NP-40, and protease inhibitors. This buffer helps break down cell membranes and release proteins for further analysis.
What is the difference between tris and tris hcl?
They are all basically the same thing. Tris-HCl is just the Tris base converted to a salt with HCl. You can buy either one. The advantage of starting with powdered Tris-HCl is that it is more soluble in water than the base and as a solution has a more neutral pH which is usually the desirable buffer point. Tris base has a pKa of over 8 so using Tris-HCl saves you the trouble of bringing it to a more neutral pH. The one thing to be careful of when making solutions from powder is to be sure to use the correct molecular weight which differs between the two. To answer your specific question, it doesn't matter which you start with except in the rare cases where the sodium from the NaOH would be an issue. For your situation where the solution is going to be slightly basic, it sounds like you could use either one as the starting reagent. I would go with whatever is already around the lab. Source link is given below.
