pH - denatures the enzyme by altering the charges on the enzyme. This will affect the structure of the enzyme and its ability to function.
Heat - will denature the enzyme by breaking its bonds. This will stop the enzyme from being able to bond to a specific substrate molecule.
Cold - not enough energy to reach minimum amount of activation energy needed to undergo reaction. The enzyme will not be able to react on the substrate.
It depends on the enzyme. Some enzymes work most efficiently in acidic solutions whereas others work best in basic solutions. E.g. Enzymes in the stomach work best at a pH level of 2 or very acidic. If they are not put in the right pH then the enzymes may not work properly/efficiently or they may denature (deactivate irreversibly)
Acids, especially strong acids, dissociate in water solutions to give off H+ ions. These H+ ions are also involved with polar bonding, R-group reactions between amino acids, and hydrophobic/hydrophilic reactions. More of these H+ ions will start bonding to different parts of the enzyme and start pulling it apart, denaturing it. Any addition of an acidic or basic solution which lowers pH by 0.5 or more will cause the enzyme to start denaturing.
Pepsin is an enzyme in the stomach that is destroyed by strong acids. Pepsin works best at an acidic pH, but too strong of an acid can denature and deactivate the enzyme.
The pH of gastric lipase is around 4.0 to 5.0, which is acidic. This acidic environment helps to activate the enzyme and facilitate its function in breaking down fats in the stomach.
Carbonic anhydrase is an enzyme that can function effectively in both acidic and basic pH environments. It plays a crucial role in maintaining the body's acid-base balance by catalyzing the reversible conversion of carbon dioxide and water to bicarbonate and protons.
It depends on the enzyme. Some enzymes work most efficiently in acidic solutions whereas others work best in basic solutions. E.g. Enzymes in the stomach work best at a pH level of 2 or very acidic. If they are not put in the right pH then the enzymes may not work properly/efficiently or they may denature (deactivate irreversibly)
Gastric Juice
The enzyme whose activity decreases in both acidic and basic environments is pepsin. Pepsin is an enzyme found in the stomach that functions optimally at a pH of around 2. In environments that are either too acidic or too basic, the structure of pepsin can be denatured, leading to a decrease in enzymatic activity.
Enzyme concentration has no effect on the rate of an enzyme-catalyzed reaction after reaching a saturation point where all enzyme active sites are occupied. At this point, adding more enzyme will not increase the reaction rate further.
Pepsin, an enzyme found in the stomach, functions best at an acidic pH because the acidic environment helps to activate and maintain its structure, allowing it to efficiently break down proteins into smaller molecules for digestion.
Each enzyme has its ideal temperature
Yes, it would effect the enzyme as i would become acidic. If it becomes too acidic then the shape of the active site will change and the substrate will no longer fit and therefore no reaction can take place. A low pH will denature the enzyme ( cause the protein structure to change shape)
Pepsin is an enzyme that functions in highly acidic conditions, specifically in the stomach where the pH can be as low as 1.5 to 3.5. It helps break down proteins into smaller peptides and amino acids for digestion.
H2SO4 is used to denature the enzyme and stop the reaction instantly. by adding H2SO4,it will prevent further reaction of the enzyme onto the substrate and the rate of enzyme reaction can be measured in the specific time
An enzyme that works best in an acidic environment would function best at a pH below 7, typically around pH 4 to 6. At this pH range, the enzyme's active site is more stable and optimal for catalyzing reactions.
Temperature
The pH range of the mouth is 5.6-6.9 slightly acidic, this is the environment in which human amylase (starch breakdown enzyme) is most active.