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The decolorizing agent in the acid fast stain is acid alcohol. The decolorizing agent in the gram stain is ethanol.

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10y ago

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Why is acid alcohol rather than ethyl alcohol used as a decolorizing agent in the acid fast staining experiment?

Alcohol is a term used for any O-H group that is attached to a carbon. Perhapes the alcohol is found in a Safranin stain. I hope I have this right but if alcohol was used as the decolorizing agent, it may wash out too much stain to get a good view in an oil emersion microscope.


Is acid fast stain a type of differential stain?

Yes, acid-fast stain is a type of differential stain.


What is the difference between ZN stain and modified ZN stain?

in Zn we use 3% decolorizer, while in modified 1% h2so4 is used, modified is used for paracite like Cryptosporidium, cyclosporidium and bacterias like nocaddia and actinomyces. also in modified heating isn't done.


Is the acid fast stain positive or negative in the sample?

The acid-fast stain is positive in the sample.


Is maneval's stain an acid stain?

Yes, Maneval's stain is an acid-fast stain used in microbiology to detect acid-fast bacteria such as Mycobacterium species. It involves using acid-alcohol to decolorize non-acid-fast bacteria while acid-fast bacteria retain the stain due to their waxy cell wall.


What is the counter or secondary stain used in the acid-fast stain technique?

The counter or secondary stain used in the acid-fast stain technique is methylene blue.


Do acid-fast bacteria stain gram-negative when subjected to the gram stain?

No, acid-fast bacteria do not stain gram-negative when subjected to the gram stain.


What are the name of cold acid fast stain?

The cold acid-fast stain used for Mycobacterium tuberculosis is called the Kinyoun stain or the Kinyoun cold stain. This staining technique is commonly used to identify acid-fast bacteria that are not easily stained by traditional methods.


Is the acid fast stain result positive or negative for the sample?

The acid-fast stain result is positive for the sample.


Is mordant used in acid fast stain?

Yes, a mordant is used in the acid-fast stain technique. The mordant used is heat to help drive the primary stain, usually carbol-fuchsin, into acid-fast bacteria, such as Mycobacterium species, which resist decolorization with acid-alcohol.


Are endospores acid fast?

Yes, endospores are acid-fast because their thick walls are resistant to most stains and dyes, including the acid-fast stain used in microbiology to detect mycobacteria like Mycobacterium tuberculosis. This resistance allows endospores to retain the stain even after decolorization with acid-alcohol.


Which color is common to both the gram stain and the acid-fast stain?

The color common to both the gram stain and the acid-fast stain is red/pink. In the gram stain, Gram-negative bacteria appear red or pink after staining with safranin, while in the acid-fast stain, acid-fast bacteria such as Mycobacterium species retain the red/pink color of carbol fuchsin despite decolorization with acid-alcohol.