The Sakaguchi reagent consists of 1-Naphthol and a drop of sodium hypobromite. The guanidine group of arginine in proteins reacts with the Sakaguchi reagent.
A positive result for the Sakaguchi test is the formation of a white or yellow precipitate in the presence of arginine or histidine in the test sample. This precipitate forms when ninhydrin (reagent used in the test) reacts with the guanidine group of arginine or the imidazole group of histidine.
The Sakaguchi test is a specific qualitative test for the detection of a specific type of protein with the amino acid containing the guanidinium group. In basic conditions, alpha naphthol and sodium hypobromite/chlorite react with the aforementioned compound to form red-orange complexes.
the amount of limiting reagent
the amount of limiting reagent
Formaldehyde Schiff's reagent: It is a solution of formaldehyde and sulfuric acid, used to detect the presence of aldehydes. The equation involves the formation of a colored compound with aldehydes. Benzaldehyde Schiff's reagent: Benzaldehyde can act as a Schiff's base with primary amines to form imines. The reaction results in the formation of a colored compound. Acetone Schiff's reagent: Acetone can react with a primary amine to form a Schiff's base, leading to the formation of a colored compound.
In the Sakaguchi test, a positive result is indicated by the formation of a red or orange color upon the addition of the Sakaguchi reagent to the sample. This reaction is used to detect the presence of certain amino acids, particularly arginine. The intensity of the color can vary depending on the concentration of the target compound in the sample.
A positive result for the Sakaguchi test is the formation of a white or yellow precipitate in the presence of arginine or histidine in the test sample. This precipitate forms when ninhydrin (reagent used in the test) reacts with the guanidine group of arginine or the imidazole group of histidine.
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Hironobu Sakaguchi email
Seiji Sakaguchi is 6' 5".
Tak Sakaguchi is 5' 7".
Hiroyuki Sakaguchi was born in 1965.
Kinichiro Sakaguchi died in 1994.
Kinichiro Sakaguchi was born in 1897.
Chikara Sakaguchi was born in 1934.
Ango Sakaguchi died in 1955.