The Sakaguchi test is a specific qualitative test for the detection of a specific type of protein with the amino acid containing the guanidinium group. In basic conditions, alpha naphthol and sodium hypobromite/chlorite react with the aforementioned compound to form red-orange complexes.
The Sakaguchi test is a chemical test that detects the presence of arginine in proteins. The guanidine in the arginine reacts with the 1-Naphthol in the reagent, forming a red-colored substance.
Reference: J. Biol. Chem., 222, (1956), 225.
A red color is the positive result>
Because this test is extremely sensitive and if there will be an in order addition of reagents, the presence of Arginine will not be sufficient to produce the desired outcome.
The Sakaguchi reagent consists of 1-Naphthol and a drop of sodium hypobromite. The guanidine group of arginine in proteins reacts with the Sakaguchi reagent.
A wet test is realized in solution.
Sakaguchi test detect arginine in proteins; the 1-naphtol with a bit of sodium hypobromite react with arginine and produce a red color complex.
Reference: J. Biol. Chem., 222, (1956), 225.
A red color is the positive result>
Ango Sakaguchi died in 1955.
Ango Sakaguchi was born in 1906.
Hiroyuki Sakaguchi was born in 1965.
Shūhei Sakaguchi was born in 1977.
Chikara Sakaguchi was born in 1934.
Kinichiro Sakaguchi was born in 1897.
Alicja Sakaguchi was born in 1954.
Seiji Sakaguchi is 6' 5".
Tak Sakaguchi is 5' 7".