The Sakaguchi test is a specific qualitative test for the detection of a specific type of protein with the amino acid containing the guanidinium group. In basic conditions, alpha naphthol and sodium hypobromite/chlorite react with the aforementioned compound to form red-orange complexes.
A positive result for the Sakaguchi test is the formation of a white or yellow precipitate in the presence of arginine or histidine in the test sample. This precipitate forms when ninhydrin (reagent used in the test) reacts with the guanidine group of arginine or the imidazole group of histidine.
The Sakaguchi test is a chemical test used to detect the presence of arginine in a sample. It involves the formation of a colored dye complex when arginine reacts with ninhydrin in an acid medium. The intensity of the color produced is proportional to the amount of arginine present in the sample.
The order of addition in Sakaguchi's test is important to prevent false positives or precipitation of chemicals. Adding α-naphthol before sulfuric acid helps in the formation of a stable color complex with guanidine, avoiding unwanted reactions that could interfere with the test results.
The Sakaguchi reagent consists of 1-Naphthol and a drop of sodium hypobromite. The guanidine group of arginine in proteins reacts with the Sakaguchi reagent.
To retake your chemistry mastery test on Platoweb, you will need to speak with your teacher or course administrator to reset the test for you. Once the test has been reset, you can then retake it following the instructions provided by your teacher or on the Platoweb platform.
Sakaguchi test detect arginine in proteins; the 1-naphtol with a bit of sodium hypobromite react with arginine and produce a red color complex.
In the Sakaguchi test, a positive result is indicated by the formation of a red or orange color upon the addition of the Sakaguchi reagent to the sample. This reaction is used to detect the presence of certain amino acids, particularly arginine. The intensity of the color can vary depending on the concentration of the target compound in the sample.
A positive result for the Sakaguchi test is the formation of a white or yellow precipitate in the presence of arginine or histidine in the test sample. This precipitate forms when ninhydrin (reagent used in the test) reacts with the guanidine group of arginine or the imidazole group of histidine.
The Sakaguchi test is a chemical test used to detect the presence of arginine in a sample. It involves the formation of a colored dye complex when arginine reacts with ninhydrin in an acid medium. The intensity of the color produced is proportional to the amount of arginine present in the sample.
Hironobu Sakaguchi email
Seiji Sakaguchi is 6' 5".
Tak Sakaguchi is 5' 7".
Hiroyuki Sakaguchi was born in 1965.
Alicja Sakaguchi was born in 1954.
Kinichiro Sakaguchi died in 1994.
Kinichiro Sakaguchi was born in 1897.
Chikara Sakaguchi was born in 1934.