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Sakaguchi test for arginine

Updated: 8/10/2023
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βˆ™ 11y ago

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Reference: J. Biol. Chem., 222, (1956), 225.

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βˆ™ 9y ago

Arginine is an amino acid. The Sakaguchi Reaction functions as a qualitative test for this substance, whether on its own or in a protein.

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Q: Sakaguchi test for arginine
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How does Sakaguchi test works?

Sakaguchi test detect arginine in proteins; the 1-naphtol with a bit of sodium hypobromite react with arginine and produce a red color complex.


Chemistry of sakaguchi test?

The Sakaguchi test is a specific qualitative test for the detection of a specific type of protein with the amino acid containing the guanidinium group. In basic conditions, alpha naphthol and sodium hypobromite/chlorite react with the aforementioned compound to form red-orange complexes.


Why should there be an order of addition of the reagents in the sakaguchi's test?

Because this test is extremely sensitive and if there will be an in order addition of reagents, the presence of Arginine will not be sufficient to produce the desired outcome.


What is the active ingredeint in sakaguchi reagent?

The Sakaguchi reagent consists of 1-Naphthol and a drop of sodium hypobromite. The guanidine group of arginine in proteins reacts with the Sakaguchi reagent.


What is the positive result for sakaguchi test?

A red color is the positive result>


What element in the amino acid is detected to be present in the lead acetate test?

Arginine.


When was Ango Sakaguchi born?

Ango Sakaguchi was born in 1906.


When was Hiroyuki Sakaguchi born?

Hiroyuki Sakaguchi was born in 1965.


When was ShΕ«hei Sakaguchi born?

Shūhei Sakaguchi was born in 1977.


When was Chikara Sakaguchi born?

Chikara Sakaguchi was born in 1934.


When was Kinichiro Sakaguchi born?

Kinichiro Sakaguchi was born in 1897.


When was Alicja Sakaguchi born?

Alicja Sakaguchi was born in 1954.