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A red color is the positive result>

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What color is positive in Sakaguchi test?

In the Sakaguchi test, a positive result is indicated by the formation of a red or orange color upon the addition of the Sakaguchi reagent to the sample. This reaction is used to detect the presence of certain amino acids, particularly arginine. The intensity of the color can vary depending on the concentration of the target compound in the sample.


What is the mechanism for sakaguchi test?

The Sakaguchi test is a colorimetric assay used to detect the presence of arginine in a sample. It involves the reaction of arginine with naphthol and hypochlorite under alkaline conditions, leading to the formation of a reddish-purple complex. This color change indicates a positive result, confirming the presence of arginine. The test is often utilized in microbiology and biochemistry for identifying specific bacterial strains.


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How does Sakaguchi test works?

Sakaguchi test detect arginine in proteins; the 1-naphtol with a bit of sodium hypobromite react with arginine and produce a red color complex.


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