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How does Sakaguchi test works?

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Anonymous

17y ago
Updated: 8/16/2019

Sakaguchi test detect arginine in proteins; the 1-naphtol with a bit of sodium hypobromite react with arginine and produce a red color complex.

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Wiki User

9y ago

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Related Questions

What color is positive in Sakaguchi test?

In the Sakaguchi test, a positive result is indicated by the formation of a red or orange color upon the addition of the Sakaguchi reagent to the sample. This reaction is used to detect the presence of certain amino acids, particularly arginine. The intensity of the color can vary depending on the concentration of the target compound in the sample.


What is the positive result for sakaguchi test?

A positive result for the Sakaguchi test is the formation of a white or yellow precipitate in the presence of arginine or histidine in the test sample. This precipitate forms when ninhydrin (reagent used in the test) reacts with the guanidine group of arginine or the imidazole group of histidine.


Sakaguchi test for arginine?

The Sakaguchi test is a chemical test used to detect the presence of arginine in a sample. It involves the formation of a colored dye complex when arginine reacts with ninhydrin in an acid medium. The intensity of the color produced is proportional to the amount of arginine present in the sample.


Chemistry of sakaguchi test?

The Sakaguchi test is a specific qualitative test for the detection of a specific type of protein with the amino acid containing the guanidinium group. In basic conditions, alpha naphthol and sodium hypobromite/chlorite react with the aforementioned compound to form red-orange complexes.


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