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1.) The 2 copies of DNA produced by replication in each chromosome remain closely associated until the cell enters prophase of mitosis. 2.) At that point, the chromosomes condense, and the two chromatids in each chromosome become clearly visible. 3.) They separate from each other in anaphase os mitosis, producing 2 cells, each with a complete set of genes coded in DNA.
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The first step of DNA replication is to copy part of the DNA sequence into?
mRNA
What is the first step of DNA replication?
1. Interphase: replication and growth of the chromosomes occur. 2. Prophase: the chromatin condenses to see the individual chromosomes and centrioles move toward the opposite poles of the cell and spindle fibers grow. 3. Metaphase: the chromosomes align in the center of the cell. 4. Anaphase: spindles attach to the chromosomes and split them in part towards the opposite poles. 5. Telophase: the cell begins to separate into to new daughter cells. 6. Cytokinesis: the nucleus begins to form and the cells are completely cut off from each other.
What major enzyme involved on replication?
DNA Helicase is the major enzyme involved in the replication of DNA. The reason why it is so important is that it unwinds the DNA which creates two separate strands.
Y shaped regions where the 2 strands of DNA separate?
It is called a replication fork.
What are the steps of DNA replication?
1)The first major step for the DNA Replication to take place is the breaking of hydrogen bonds between bases of the two antiparallel strands. The unwounding of the two strands is the starting point. The splitting happens in places of the chains which are rich in A-T. That is because there are only two bonds between Adenine and Thymine (there are three hydrogen bonds between Cytosine and Guanine). Helicase is the enzyme that splits the two strands. The initiation point where the splitting starts is called "origin of replication".The structure that is created is known as "Replication Fork".2) One of the most important steps of DNA Replication is the binding of RNA Primase in the the initiation point of the 3'-5' parent chain. RNA Primase can attract RNA nucleotides which bind to the DNA nucleotides of the 3'-5' strand due to the hydrogen bonds between the bases. RNA nucleotides are the primers (starters) for the binding of DNA nucleotides.3) The elongation process is different for the 5'-3' and 3'-5' template.a)5'-3' Template: The 3'-5' proceeding daughter strand -that uses a 5'-3' template- is called leading strandbecause DNA Polymerase ä can "read" the template and continuously adds nucleotides (complementary to the nucleotides of the template, for example Adenine opposite to Thymine etc).b)3'-5'Template: The 3'-5' template cannot be "read" by DNA Polymerase ä. The replication of this template is complicated and the new strand is called lagging strand. In the lagging strand the RNA Primase adds more RNA Primers. DNA polymerase å reads the template and lengthens the bursts. The gap between two RNA primers is called "Okazaki Fragments".The RNA Primers are necessary for DNA Polymerase å to bind Nucleotides to the 3' end of them. The daughter strand is elongated with the binding of more DNA nucleotides.4) In the lagging strand the DNA Pol I-exonuclease- reads the fragments and removes the RNA Primers. The gaps are closed with the action of DNA Polymerase (adds complementary nucleotides to the gaps) and DNA Ligase (adds phosphate in the remaining gaps of the phosphate - sugar backbone).Each new double helix is consisted of one old and one new chain. This is what we call semiconservative replication.5) The last step of DNA Replication is the Termination. This process happens when the DNA Polymerase reaches to an end of the strands. We can easily understand that in the last section of the lagging strand, when the RNA primer is removed, it is not possible for the DNA Polymerase to seal the gap (because there is no primer). So, the end of the parental strand where the last primer binds isn't replicated. These ends of linear (chromosomal) DNA consists of noncoding DNA that contains repeat sequences and are called telomeres. As a result, a part of the telomere is removed in every cycle of DNA Replication.6) The DNA Replication is not completed before a mechanism of repair fixes possible errors caused during the replication. Enzymes like nucleases remove the wrong nucleotides and the DNA Polymerase fills the gaps.Similar processes also happen during the steps of DNA Replication of prokaryotes though there are some differences.
Difference between eukaryotic DNA replication and bacterial replication?
The difference between Eukaryotic DNA and bacterial genome replication is the eukaryotic DNA is mostly linear and has multiple sites of replication. They both are bidirectional.
What the first step in the replication of DNA the?
mitosis is the very first step in DNA replication my dear friend
What is the first step in replication?
The first step to identifying DNA is to Determine the cell
What is the first step of replication?
The first, and potentially most important, step of DNA replication is unzipping the DNA through enzymes. This allows more nucleotides to be attached to the halves of the DNA, to create more.
The first step of DNA replication is to copy part of the DNA sequence into?
mRNA
Which is the first step to occur during the process of replication?
Separation of the two DNA strands by the action of the Helicase enzyme.
Compare the number of replication forks in prokaryotic and eukaryotic DNA during replication?
Prokaryotic DNA is reproduced with DNA Polymerases replication forks.
What is the helicase enzyme?
Unwinds the DNA during DNA replication and Trancription
There is just a single origin of replication in bacterial DNA whereas there are hundreds or perhaps thousands of origins of replication in the DNA of eukaryotic organisms?
true
In the eukaryotic cell where does DNA replication occur?
it occurs in the nucleus...
How does DNA replication differ in prokaryotes and eukaryotes?
1. In eukaryotic cells replication forks make several start sites along the DNA strand which forms replication "bubbles" which get larger the more DNA is copied, and stop when DNA replication is complete. In prokaryotic cell's DNA is formed in a loop, two replication forks start along one part of the loop (origin replication) and the replication forks copy DNA in opposite directions until they meet at the other side of the loop, making an exact copy of DNA.
Where are Nucleotides used for DNA replication in eukaryotic cells made?
nucleus... I believe
