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Why is the wavelength of light set at maximum absorbance when making a Beer's Law plot?
The wavelength of light is set at maximum absorbance when making a Beer's Law plot because it allows for the most accurate and precise measurement of the absorbance of a sample. This wavelength corresponds to the peak absorbance of the sample, providing the most reliable data for constructing the plot and determining the concentration of the analyte.
What is the absorbance spectrum of the given compound and where does the lambda max fall on the spectrum?
The absorbance spectrum of a compound shows how much light it absorbs at different wavelengths. The lambda max, or maximum absorbance, is the point on the spectrum where the compound absorbs the most light.
What is difference between specific absorbance and absorbance interms of spectroscopy?
specific absorbance- it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell. so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light. divya.chakraborty@gmail.com
Does Amax of bromophenol blue vary with concentration?
Yes, the absorbance maximum (Amax) of bromophenol blue does vary with concentration. As the concentration of bromophenol blue increases, the Amax shifts from its initial value. This change in Amax can be used to determine the concentration of bromophenol blue in a solution through spectrophotometric analysis.
Is absorbance considered a discrete or continuous variable?
Absorbance is considered a continuous variable.
Why do you use the wavelength with the maximum absorbance in spectroscop?
The wavelength with the maximum absorbance corresponds to the peak absorption of the compound being analyzed, providing the most accurate and precise measurement. By measuring absorbance at the maximum wavelength, we can ensure the highest sensitivity and specificity in detecting and quantifying the compound of interest.
What is maximum wavelength of absorbance of sodium dichromate?
The maximum wavelength of absorbance for sodium dichromate typically occurs around 350-370 nanometers (nm). This absorbance is primarily due to the presence of the chromate ion, which exhibits strong UV-visible absorbance characteristics. The specific wavelength can vary slightly depending on the concentration and the solvent used.
Why is the wavelength of light set at maximum absorbance when making a Beer's Law plot?
The wavelength of light is set at maximum absorbance when making a Beer's Law plot because it allows for the most accurate and precise measurement of the absorbance of a sample. This wavelength corresponds to the peak absorbance of the sample, providing the most reliable data for constructing the plot and determining the concentration of the analyte.
What is maximum absorbance of methyle orange at 242 nm?
The maximum absorbance of methyl orange typically occurs at around 464 nm, not 242 nm. At 242 nm, the absorbance may be lower or not significant, as this wavelength is outside the main absorption range for methyl orange. For accurate absorbance values, it is important to refer to specific absorption spectra or experimental data for methyl orange.
What is the specific absorbance of aspirin?
If you have a spectrofotometer ( the thing to mesure the absorbance) then play with the setting and use a maximum. this will lay close to your specific absorbance or take the pharmacopea or a MERCK index
What is another name of betacarotene?
Betacarotene has many other names - the one most recognised is orange 5 in food.
What is the absorbance spectrum of the given compound and where does the lambda max fall on the spectrum?
The absorbance spectrum of a compound shows how much light it absorbs at different wavelengths. The lambda max, or maximum absorbance, is the point on the spectrum where the compound absorbs the most light.
Why absorbance is decreased after maximum wavelength achieved?
After reaching maximum absorbance at a certain wavelength, further increase in wavelength leads to decreased absorbance because the molecules are not absorbing light at those wavelengths as efficiently. This decrease may be attributed to a shift in the electronic energy levels of the molecules, causing them to absorb less light as the wavelength increases beyond the maximum.
Why do you use the wavelength with the maximum absorbance in spectroscopy?
Short answer:Using the maximum wavelength gives us the best results. This is because at the peak absorbance, the absobance strength of light will be at the highest and rate of change in absorbance with wavelength will be the smallest. Measurements made at the peak absorbance will have the smallest error.Long answer: It really depends on what is the largest source of error. Taking the readings at the peak maximum is best at low absorbance, because it gives the best signal-to-noise ratio, which improves the precision of measurement. If the dominant source of noise is photon noise, the precision of absorbance measurement is theoretically best when the absorbance is near 1.0. So if the peak absorbance is below 1.0, then using the peak wavelength is best, but if the peak absorbance is well above 1.0, you might be better off using another wavelength where the absorbance is closer to 1. Another issue is calibration curve non-linearity, which can result in curve-fitting errors. The non-linearity caused by polychromatic light is minimized if you take readings at either a peak maximum or a minimum, because the absorbance change with wavelength is the smallest at those wavelengths. On the other hand, using the maximum increases the calibration curve non-linearity caused by stray light. Very high absorbances cause two problems: the precision of measurement is poor because the transmitted intensity is so low, and the calibration curve linearity is poor due to stray light. The effect of stray light can be reduced by taking the readings at awavelength where the absorbance is lower or by using a non-linear calibration curve fitting technique. Finally, if spectral interferences are a problem, the best measurement wavelength may be the one that minimizes the relative contribution of spectral interferences (which may or may not be the peak maximum). In any case, don't forget: whatever wavelength you use, you have to use the exact same wavelength for all the standards and samples. See http://terpconnect.umd.edu/~toh/models/BeersLaw.htmlTom O'HaverProfessor Emeritus
What are the white hairs on carrots?
They lack the basic [betacarotene] pigment.
What is maximum wavelength of absorbance of potassium permanganate?
The maximum wavelength of absorbance for potassium permanganate is around 525 nanometers. At this wavelength, potassium permanganate exhibits a strong absorption peak due to its characteristic deep purple color. This absorption peak is often used in spectrophotometric analyses to quantify the concentration of potassium permanganate in solution.
Is wavelength or absorbance the dependent variable?
"absorbance"Since in the experiment, you probably choose the wavelength, then measure the absorbance (absorption?, the absorbance is the dependent variable.
